State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, Liaoning 116034, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian, Liaoning 116034, China; Dalian Key Laboratory for Precision Nutrition, Dalian Polytechnic University, Dalian, Liaoning 116034, China; Dalian Jinshiwan Laboratory, Dalian, Liaoning 116034, China.
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt; Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum, Turkey.
Colloids Surf B Biointerfaces. 2024 Jul;239:113903. doi: 10.1016/j.colsurfb.2024.113903. Epub 2024 Apr 6.
Nicotinamide mononucleotide (NMN) is being investigated for its ability to address the decline in NAD level during aging. This study aimed to construct a delivery system based on ovalbumin and fucoidan nanoparticles to ameliorate the bioaccessibility of NMN by increasing NAD level in aging mouse model. The NMN-loaded ovalbumin and fucoidan nanoparticles (OFNPs) were about 177 nm formed by the interplay of hydrogen bonds between ovalbumin and fucoidan. Compared with free NMN, NMN-loaded OFNPs intervention could obviously improve the antioxidant enzyme activity of senescent cell induced by D-galactose. The NMN-loaded OFNPs treatment could ameliorate the loss of weight and organ index induced by senescence, and maintain the water content for the aging mice. The Morris maze test indicated that hitting blind side frequency and escape time of NMN-loaded OFNPs group decreased by 13% and 35% compared with that of free NMN group. Furthermore, the NMN-loaded OFNPs significantly alleviated the age-related oxidative stress and increased the generation of NAD 1.34 times by improving the bioaccessibility of NMN. Our data in this study supplied a strategy to enhance the bioavailability of NMN in senescence treatment.
烟酰胺单核苷酸 (NMN) 因其能够提高衰老过程中 NAD 水平的能力而受到关注。本研究旨在构建基于卵清蛋白和褐藻糖胶纳米粒子的递送系统，通过增加衰老小鼠模型中的 NAD 水平来提高 NMN 的生物利用度。NMN 负载的卵清蛋白和褐藻糖胶纳米粒子（OFNPs）由卵清蛋白和褐藻糖胶之间氢键的相互作用形成，约为 177nm。与游离 NMN 相比，NMN 负载的 OFNPs 干预可以明显提高 D-半乳糖诱导衰老细胞的抗氧化酶活性。NMN 负载的 OFNPs 处理可以改善衰老引起的体重和器官指数的损失，并维持衰老小鼠的水分含量。Morris 水迷宫试验表明，NMN 负载的 OFNPs 组的盲目侧撞击频率和逃逸时间分别比游离 NMN 组降低了 13%和 35%。此外，NMN 负载的 OFNPs 通过提高 NMN 的生物利用度，显著缓解了与年龄相关的氧化应激，并将 NAD 的产生增加了 1.34 倍。我们在这项研究中的数据为提高 NMN 在衰老治疗中的生物利用度提供了一种策略。
