Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt.
Obstetrics and Gynecology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Inflammation. 2023 Aug;46(4):1192-1208. doi: 10.1007/s10753-023-01799-1. Epub 2023 Mar 31.
The study included 32 women with PAS and 20 with normally implanted placenta as a control group. Vascular endothelial cell growth factor (VEGF), Soluble FMS Like Tyrosine Kinase (sFLT-1/sVEGFR1), and Endoglin (ENG) were measured in placenta tissue by ELISA. Granzyme B (GrzB) expression in trophoblastic and stromal mesenchymal cells was evaluated by immunohistochemistry. MAIT, NK, and NKT cells were assessed in blood and placenta by flow cytometry. Alterations were observed in levels of MAIT cells, NK cell subsets, and NKT cells in patients compared with controls. Several significant correlations were detected between these cells and GrzB scores, VEGF, ENG, and sFLT-1 levels. This is the first study analysing these cells in PAS patients and correlating their levels with changes in some angiogenic and antiangiogenic factors implicated in trophoblast invasion and with GrzB distribution in trophoblast and stroma. Interrelation between these cells probably plays an important role in pathogenesis of PAS.
该研究纳入了 32 名 PAS 患者和 20 名正常胎盘植入患者作为对照组。通过 ELISA 法检测胎盘组织中血管内皮生长因子（VEGF）、可溶性 FMS 样酪氨酸激酶（sFLT-1/sVEGFR1）和内皮糖蛋白（ENG）。通过免疫组织化学法评估滋养层和间质间充质细胞中颗粒酶 B（GrzB）的表达。通过流式细胞术评估血液和胎盘中的 MAIT、NK 和 NKT 细胞。与对照组相比，患者的 MAIT 细胞、NK 细胞亚群和 NKT 细胞水平发生了变化。这些细胞与 GrzB 评分、VEGF、ENG 和 sFLT-1 水平之间存在一些显著相关性。这是首次分析 PAS 患者这些细胞并将其水平与参与滋养细胞侵袭的一些血管生成和抗血管生成因子的变化以及滋养层和间质中 GrzB 分布相关联的研究。这些细胞之间的相互关系可能在 PAS 的发病机制中发挥重要作用。
