Yang Jihoon, Sooksa-Nguan Thanwalee, Kannan Baskaran, Cano-Alfanar Sofia, Liu Hui, Kent Angela, Shanklin John, Altpeter Fredy, Howe Adina
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA, USA.
DOE Center for Advanced Bioenergy and Bioproducts Innovation, Ames, IA, USA.
Biotechnol Biofuels Bioprod. 2023 Mar 30;16(1):56. doi: 10.1186/s13068-023-02302-6.
Oilcane is a metabolically engineered sugarcane (Saccharum spp. hybrid) that hyper-accumulates lipids in its vegetable biomass to provide an advanced feedstock for biodiesel production. The potential impact of hyper-accumulation of lipids in vegetable biomass on microbiomes and the consequences of altered microbiomes on plant growth and lipid accumulation have not been explored so far. Here, we explore differences in the microbiome structure of different oilcane accessions and non-modified sugarcane. 16S SSU rRNA and ITS rRNA amplicon sequencing were performed to compare the characteristics of the microbiome structure from different plant compartments (leaf, stem, root, rhizosphere, and bulk soil) of four greenhouse-grown oilcane accessions and non-modified sugarcane. Significant differences were only observed in the bacterial microbiomes. In leaf and stem microbiomes, more than 90% of the entire microbiome of non-modified sugarcane and oilcane was dominated by similar core taxa. Taxa associated with Proteobacteria led to differences in the non-modified sugarcane and oilcane microbiome structure. While differences were observed between multiple accessions, accession 1566 was notable in that it was consistently observed to differ in its microbial membership than other accessions and had the lowest abundance of taxa associated with plant-growth-promoting bacteria. Accession 1566 is also unique among oilcane accessions in that it has the highest constitutive expression of the WRI1 transgene. The WRI1 transcription factor is known to contribute to significant changes in the global gene expression profile, impacting plant fatty acid biosynthesis and photomorphogenesis. This study reveals for the first time that genetically modified oilcanes associate with distinct microbiomes. Our findings suggest potential relationships between core taxa, biomass yield, and TAG in oilcane accessions and support further research on the relationship between plant genotypes and their microbiomes.
油甘蔗是一种经过代谢工程改造的甘蔗（甘蔗属杂交种），其植物生物质中脂质超积累，可为生物柴油生产提供优质原料。到目前为止，尚未探究植物生物质中脂质超积累对微生物群落的潜在影响以及微生物群落改变对植物生长和脂质积累的后果。在此，我们探究了不同油甘蔗品种和未改良甘蔗的微生物群落结构差异。进行了16S SSU rRNA和ITS rRNA扩增子测序，以比较四种温室种植的油甘蔗品种和未改良甘蔗不同植物部位（叶、茎、根、根际和大田土壤）的微生物群落结构特征。仅在细菌微生物群落中观察到显著差异。在叶和茎微生物群落中，未改良甘蔗和油甘蔗的整个微生物群落中超过90%由相似的核心分类群主导。与变形菌门相关的分类群导致了未改良甘蔗和油甘蔗微生物群落结构的差异。虽然在多个品种之间观察到了差异，但1566品种值得注意，因为与其他品种相比，始终观察到其微生物组成不同，且与促进植物生长的细菌相关的分类群丰度最低。1566品种在油甘蔗品种中也很独特，因为它具有WRI1转基因的最高组成型表达。已知WRI1转录因子会导致全球基因表达谱发生显著变化，影响植物脂肪酸生物合成和光形态建成。本研究首次揭示了转基因油甘蔗与不同的微生物群落相关联。我们的研究结果表明了油甘蔗品种中核心分类群、生物量产量和三酰甘油之间的潜在关系，并支持进一步研究植物基因型与其微生物群落之间的关系。
