Novaković Lazar, Yakubov Gleb E, Ma Yingxuan, Bacic Antony, Blank Kerstin G, Sampathkumar Arun, Johnson Kim L
School of BioSciences, University of Melbourne, Parkville, VIC, Australia.
School of Biosciences, Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
Front Plant Sci. 2023 Mar 14;14:1150202. doi: 10.3389/fpls.2023.1150202. eCollection 2023.
The cell wall is one of the defining features of plants, controlling cell shape, regulating growth dynamics and hydraulic conductivity, as well as mediating plants interactions with both the external and internal environments. Here we report that a putative mechanosensitive Cys-protease DEFECTIVE KERNEL1 (DEK1) influences the mechanical properties of primary cell walls and regulation of cellulose synthesis. Our results indicate that DEK1 is an important regulator of cellulose synthesis in epidermal tissue of cotyledons during early post-embryonic development. DEK1 is involved in regulation of cellulose synthase complexes (CSCs) by modifying their biosynthetic properties, possibly through interactions with various cellulose synthase regulatory proteins. Mechanical properties of the primary cell wall are altered in modulated lines with DEK1 affecting both cell wall stiffness and the thickness of the cellulose microfibril bundles in epidermal cell walls of cotyledons.
细胞壁是植物的标志性特征之一，它控制细胞形状、调节生长动态和水力传导率，还介导植物与外部和内部环境的相互作用。在此，我们报告一种假定的机械敏感半胱氨酸蛋白酶——缺陷胚乳1（DEK1）影响初生细胞壁的机械性能和纤维素合成的调控。我们的结果表明，DEK1是胚后早期发育期间子叶表皮组织中纤维素合成的重要调节因子。DEK1可能通过与各种纤维素合酶调节蛋白相互作用，改变纤维素合酶复合体（CSC）的生物合成特性，从而参与对其的调控。在DEK1表达被调节的株系中，初生细胞壁的机械性能发生改变，这影响了子叶表皮细胞壁的硬度和纤维素微纤丝束的厚度。
