Yamada K, Sasaki M, Kimura G
In Vitro Cell Dev Biol. 1986 Apr;22(4):212-6. doi: 10.1007/BF02623305.
We examined cellular protein content in four temperature-sensitive (ts) mutants of rat 3Y1 fibroblasts (3Y1tsD123, 3Y1tsF121, 3Y1tsG125, and 3Y1tsH203) under various conditions of culture that affect cell proliferation. When proliferation of the ts mutants was inhibited at a nonpermissive temperature (39.8 degrees C) in the G1 phase, prominent accumulation of cellular protein occurred in three mutants (3Y1tsF121, 3Y1tsG125, and 3Y1tsH203) but not in 3Y1tsD123. The over-accumulation of protein at 39.8 degrees C in the former three mutants was inhibited at high cell densities. At low cell densities there was an upper limit in the protein accumulation at 39.8 degrees C. When the three mutants, proliferation-arrested at high cell densities at 33.8 degrees C, were replated sparsely in fresh medium and shifted to 39.8 degrees C, proliferation was completely inhibited, whereas over-accumulation of protein occurred. These results indicating dissociation of protein accumulation and cell proliferation suggest that the two events are regulated by different mechanisms.
我们检测了大鼠3Y1成纤维细胞的四个温度敏感（ts）突变体（3Y1tsD123、3Y1tsF121、3Y1tsG125和3Y1tsH203）在影响细胞增殖的各种培养条件下的细胞蛋白质含量。当ts突变体在G1期于非允许温度（39.8℃）下增殖受到抑制时，三个突变体（3Y1tsF121、3Y1tsG125和3Y1tsH203）出现了明显的细胞蛋白质积累，而3Y1tsD123中未出现。前三个突变体在39.8℃时蛋白质的过度积累在高细胞密度下受到抑制。在低细胞密度时，39.8℃下蛋白质积累存在上限。当这三个在33.8℃下高细胞密度时增殖被阻滞的突变体在新鲜培养基中稀疏传代并转移至39.8℃时，增殖被完全抑制，而蛋白质出现过度积累。这些表明蛋白质积累与细胞增殖分离的结果提示这两个事件受不同机制调控。
