Wren F E, Schor A M, Schor S L, Grant M E
J Cell Physiol. 1986 May;127(2):297-302. doi: 10.1002/jcp.1041270217.
We have studied the combined effects of platelet-derived soluble factors and three types of macromolecular substrata on the proliferation and migration of smooth muscle cells in vitro. Bovine aortic smooth muscle cells were plated onto three-dimensional gels of type I collagen or onto cell-free extracellular matrices deposited on such gels by either bovine aortic endothelial cells or smooth muscle cells. The cells were cultured in the presence of whole-blood serum (WBS) or platelet-poor plasma (PPP). Smooth muscle cell proliferation on type I collagen gels was dependent on the presence of platelet-derived factors, i.e. the cells proliferated in the presence of WBS but not in PPP. In contrast, cell proliferation on the extracellular matrices occurred at the same rate in PPP and WBS. Smooth muscle cells plated onto collagen gels rapidly migrated down into the gel matrix; the percentage of cells migrating was inversely proportional to cell density. The presence of extracellular matrices did not alter the rate of cell migration into the underlying gel matrix. Irrespective of the substratum used, smooth muscle cell migration was independent of platelet-derived or plasma factors and occurred in the absence of proliferation. These results indicate that possible chemotactic, chemokinetic, and/or mitogenic factors produced by the vascular cells and deposited within the extracellular matrix may play an important role in modulating smooth muscle cell behaviour in the vascular wall.
我们研究了血小板衍生的可溶性因子和三种类型的大分子基质对体外平滑肌细胞增殖和迁移的联合作用。将牛主动脉平滑肌细胞接种到I型胶原蛋白的三维凝胶上，或接种到由牛主动脉内皮细胞或平滑肌细胞沉积在这种凝胶上的无细胞细胞外基质上。细胞在全血血清（WBS）或乏血小板血浆（PPP）存在的情况下进行培养。I型胶原蛋白凝胶上的平滑肌细胞增殖依赖于血小板衍生因子的存在，即细胞在WBS存在下增殖，但在PPP中不增殖。相反，细胞在细胞外基质上的增殖在PPP和WBS中以相同的速率发生。接种到胶原蛋白凝胶上的平滑肌细胞迅速向下迁移到凝胶基质中；迁移细胞的百分比与细胞密度成反比。细胞外基质的存在并没有改变细胞迁移到下面凝胶基质中的速率。无论使用何种基质，平滑肌细胞的迁移都与血小板衍生或血浆因子无关，并且在没有增殖的情况下发生。这些结果表明，血管细胞产生并沉积在细胞外基质中的可能的趋化、趋动和/或促有丝分裂因子可能在调节血管壁中平滑肌细胞行为方面发挥重要作用。
