Univ Rouen Normandie, Laboratoire COBRA (UMR 6014 & FR 3038), INSA de Rouen, CNRS, F-76000 Rouen, France; Univ Rouen Normandie, Laboratoire Glyco-MEV UR 4358, SFR Normandie Végétal FED 4277, F-76000 Rouen, France.
Univ Rouen Normandie, Laboratoire COBRA (UMR 6014 & FR 3038), INSA de Rouen, CNRS, F-76000 Rouen, France.
Plant Sci. 2023 Jun;331:111694. doi: 10.1016/j.plantsci.2023.111694. Epub 2023 Mar 31.
Large amounts of root exudates are released by plant roots into the soil. Due to their importance in regulating the rhizosphere properties, it is necessary to unravel the precise composition and function of exudates at the root-soil interface. However, obtaining root exudates without inducing artefacts is a difficult task. To analyse the low molecular weight molecules secreted by pea roots, a protocol of root exudate collection was developed to perform a metabolomics analysis using Nuclear Magnetic Resonance (NMR). To date a few NMR studies are dedicated to root exudates. Plant culture, exudates collection and sample preparation methods had thus to be adapted to the NMR approach. Here, pea seedlings were hydroponically grown. The obtained NMR fingerprints show that osmotic stress increases the quantity of the exudates but not their diversity. We therefore selected a protocol reducing the harvest time and using an ionic solvent and applied it to the analysis of faba bean exudates. NMR analysis of the metabolic profiles allowed to discriminate between pea and faba bean according to their exudate composition. This protocol is therefore very promising for studying the composition of root exudates from different plant species as well as their evolution in response to different environmental conditions or pathophysiological events.
大量的根系分泌物被植物根系释放到土壤中。由于它们在调节根际特性方面的重要性，因此有必要揭示根系-土壤界面中分泌物的精确组成和功能。然而，在不引入人为因素的情况下获取根系分泌物是一项艰巨的任务。为了分析豌豆根系分泌的低分子量分子，我们开发了一种根系分泌物收集方案，以便使用核磁共振（NMR）进行代谢组学分析。迄今为止，只有少数几项 NMR 研究致力于根系分泌物。因此，植物培养、分泌物收集和样品制备方法必须适应 NMR 方法。在这里，豌豆幼苗在水培中生长。获得的 NMR 指纹图谱显示，渗透胁迫会增加分泌物的数量，但不会改变其多样性。因此，我们选择了一种减少收获时间并使用离子溶剂的方案，并将其应用于分析野豌豆分泌物。代谢谱的 NMR 分析能够根据分泌物组成区分豌豆和野豌豆。因此，该方案非常有前途，可以用于研究不同植物物种的根系分泌物组成及其对不同环境条件或病理生理事件的反应。
