De Croo F, Van den Bossche W, De Moerloose P
J Chromatogr. 1986 Feb 28;354:367-73. doi: 10.1016/s0021-9673(01)87037-0.
A procedure for the simultaneous quantitative determination and selective identification of potassium canrenoate and butizide is proposed. The amount of canrenone, the degradation product of potassium canrenoate, is also determined. Elution of these compounds is investigated on LiChrosorb RP-18 and RP-8 columns. The analysis time was shorter on a RP-8 column while the specific identification was still possible. Therefore, the LiChrosorb RP-8 column was chosen. The eluent consisted of an acetonitrile--0.05 M phosphate buffer, pH 4 (45:55) mixture. Quantitative determination was performed at the absorption maximum of each compound: 286 nm for potassium canrenoate and for canrenone, 271 nm for butizide. This change in wavelength is performed automatically by the computer that controls the spectrophotometric diode array detector.
提出了一种同时定量测定和选择性鉴定坎利酸钾和布噻嗪的方法。还测定了坎利酸钾的降解产物坎利酮的含量。研究了这些化合物在LiChrosorb RP - 18和RP - 8柱上的洗脱情况。在RP - 8柱上分析时间较短，同时仍可进行特异性鉴定。因此，选择了LiChrosorb RP - 8柱。洗脱液由乙腈 - 0.05 M磷酸盐缓冲液（pH 4，45:55）混合物组成。在每种化合物的吸收最大值处进行定量测定：坎利酸钾和坎利酮为286 nm，布噻嗪为271 nm。波长的这种变化由控制分光光度二极管阵列检测器的计算机自动完成。
