Department of Chemical Engineering, Faculty of Engineering, Razi University, Kermanshah, Iran.
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
J Food Sci. 2023 May;88(5):2090-2103. doi: 10.1111/1750-3841.16553. Epub 2023 Apr 2.
This study investigates the fabrication of films based on a polymer containing nanoclay, sodium diacetate (SDA), and tert-butyl hydroquinone (TBHQ) for packaging rainbow trout fillets. The films were prepared by the addition of 2% SDA (SDA film), 2% TBHQ (TBHQ film), and a combination of both (1% SDA + 1% TBHQ) into polyethylene polymer (93.00%) and montmorillonite nanoclay (5.00%). A film lack of nanoclay, SDA, and TBHQ was prepared and considered a control film. A film was also prepared by the addition of 95 g polyethylene + 5 g nanoclay (Nanoclay). Morphological properties of the films were investigated by a scanning electron microscope (SEM). In vitro antioxidant properties and antibacterial activities of the films and their effects as the coating on fish samples were evaluated against Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli. The effects of films on oxidative stability, antibacterial activity, pH, total volatile basic nitrogen (TVBN), and total viable count (TVC) of fish samples were assessed. The SEM results showed the homogenous dispersion of SDA and TBHQ into films. The SDA, TBHQ, and ST films showed antibacterial activity against L. monocytogenes, S. typhimurium, and E. coli compared with the control film as the coating and under in vitro conditions (p < 0.05). The TBHQ and ST films exhibited higher antioxidant activity and prevented the oxidation as the coating. The films prepared from the SDA, TBHQ, and ST prevented an increase in TVC and TVBN (p < 0.05). The ST films can prevent spoilage in fish samples and can be utilized in the food industry. PRACTICAL APPLICATION: We successfully prepared films with the help of nanoclay, sodium diacetate (SDA), and tert-butyl hydroquinone (TBHQ) on polyethylene for packaging fish fillets. Films containing SDA, TBHQ, and nanoclay showed antibacterial activity and prevented spoilage. The films can be used for packaging fish fillets.
本研究旨在制备一种基于含有纳米粘土、双乙酸钠（SDA）和叔丁基对苯二酚（TBHQ）的聚合物的薄膜，用于包装彩虹鳟鱼片。通过向聚乙烯聚合物（93.00%）和蒙脱土纳米粘土（5.00%）中添加 2%的 SDA（SDA 薄膜）、2%的 TBHQ（TBHQ 薄膜）和两者的组合（1%的 SDA+1%的 TBHQ）来制备薄膜。制备了一种不含纳米粘土、SDA 和 TBHQ 的薄膜作为对照薄膜。还通过添加 95g 聚乙烯+5g 纳米粘土（纳米粘土）制备了一种薄膜。通过扫描电子显微镜（SEM）研究了薄膜的形态特性。评估了薄膜的体外抗氧化特性和抗菌活性及其作为鱼样涂层的效果，以对抗单核细胞增生李斯特菌、肠炎沙门氏菌和大肠杆菌。评估了薄膜对鱼样的氧化稳定性、抗菌活性、pH 值、总挥发性碱性氮（TVBN）和总活菌数（TVC）的影响。SEM 结果表明 SDA 和 TBHQ 均匀分散在薄膜中。与对照薄膜相比，SDA、TBHQ 和 ST 薄膜作为涂层，在体外条件下对单核细胞增生李斯特菌、肠炎沙门氏菌和大肠杆菌均具有抗菌活性（p<0.05）。TBHQ 和 ST 薄膜表现出更高的抗氧化活性，并防止了氧化作为涂层。SDA、TBHQ 和 ST 制备的薄膜防止了 TVC 和 TVBN 的增加（p<0.05）。ST 薄膜可以防止鱼样变质，可以在食品工业中使用。实际应用：我们成功地在聚乙烯上制备了含有纳米粘土、双乙酸钠（SDA）和叔丁基对苯二酚（TBHQ）的薄膜，用于包装鱼片。含有 SDA、TBHQ 和纳米粘土的薄膜具有抗菌活性，可防止变质。这些薄膜可用于包装鱼片。
