采用柱液相色谱法对血液和血浆中的阿西美辛及其代谢产物吲哚美辛进行定量测定。
Quantitative determination of acemetacin and its metabolite indometacin in blood and plasma by column liquid chromatography.
作者信息
Schöllnhammer G, Dell H D, Doersing K, Kamp R
出版信息
J Chromatogr. 1986 Mar 7;375(2):331-8. doi: 10.1016/s0378-4347(00)83725-1.
A column liquid chromatographic (LC) method using UV detection for the determination of acemetacin and its metabolite indometacin in blood is described. The lower detection limit for both compounds is ca. 25 micrograms/l, the precision (coefficient of variation) is 6% for acemetacin and 10% for indometacin. The method is also suited for determination of both compounds in plasma, precisions in this case are even better than for blood, i.e. around 3% for both acemetacin and indometacin. Blood samples of three volunteers who had received 90 mg of acemetacin orally were analysed using the new method and very good agreement with results from a thin-layer chromatographic/fluorescence method was found.
描述了一种使用紫外检测的柱液相色谱（LC）方法，用于测定血液中的阿西美辛及其代谢物吲哚美辛。两种化合物的最低检测限约为25微克/升，阿西美辛的精密度（变异系数）为6%，吲哚美辛为10%。该方法也适用于测定血浆中的两种化合物，在这种情况下，精密度甚至比血液中的更好，即阿西美辛和吲哚美辛均约为3%。使用新方法分析了三名口服90毫克阿西美辛的志愿者的血样，发现与薄层色谱/荧光法的结果非常吻合。
Zotero 插件