Sohn Eun Jung
College of Medicine, Pusan National University, Yangsan 50612, Republic of Korea.
Genet Res (Camb). 2023 Mar 23;2023:8511036. doi: 10.1155/2023/8511036. eCollection 2023.
The outbreak of monkeypox may be considered a novel and urgent threat after the coronavirus disease (COVID-19). No wide-ranging studies have been conducted on this disease since it was first reported. We systematically assessed the functional role of gene expression in cells infected with the monkeypox virus using transcriptome profiling and compared the functional relation with that of COVID-19. Based on the Gene Expression Omnibus database, we obtained 212 differentially expressed genes (DEGs) of GSE36854 and GSE21001 of monkeypox datasets. Enrichment analyses, including KEGG and gene ontology (GO) analyses, were performed to identify the common function of 212 DEGs of GSE36854 and GSE21001. CytoHubba and Molecular Complex Detection were performed to determine the core genes after a protein-protein interaction (PPI). Metascape/COVID-19 was used to compare DEGs of monkeypox and COVID-19. GO analysis of 212 DEGs of GSE36854 and GSE21001 for monkeypox infection showed cellular response to cytokine stimulus, cell activation, and cell differentiation regulation. KEGG analysis of 212 DEGs of GSE36854 and GSE21001 for monkeypox infection showed involvement of monkeypox in COVID-19, cytokine-cytokine receptor interaction, inflammatory bowel disease, atherosclerosis, TNF signaling, and T cell receptor signaling. By comparing our data with published transcriptome of severe acute respiratory syndrome coronavirus 2 infections in other cell lines, the common function of monkeypox and COVID-19 includes cytokine signaling in the immune system, TNF signaling, and MAPK cascade regulation. Thus, our data suggest that the molecular connections identified between COVID-19 and monkeypox elucidate the causes of monkeypox.
猴痘疫情可能被视为继冠状病毒病（COVID-19）之后的一种新的紧迫威胁。自该疾病首次报告以来，尚未对其进行广泛研究。我们使用转录组分析系统评估了感染猴痘病毒的细胞中基因表达的功能作用，并将其功能关系与COVID-19进行了比较。基于基因表达综合数据库，我们获得了猴痘数据集GSE36854和GSE21001的212个差异表达基因（DEG）。进行了包括KEGG和基因本体（GO）分析在内的富集分析，以确定GSE36854和GSE21001的212个DEG的共同功能。在蛋白质-蛋白质相互作用（PPI）之后，使用CytoHubba和分子复合物检测来确定核心基因。使用Metascape/COVID-19比较猴痘和COVID-19的DEG。对GSE36854和GSE21001的212个DEG进行猴痘感染的GO分析显示，细胞对细胞因子刺激、细胞活化和细胞分化调节有反应。对GSE36854和GSE21001的212个DEG进行猴痘感染的KEGG分析显示，猴痘参与了COVID-19、细胞因子-细胞因子受体相互作用、炎症性肠病、动脉粥样硬化、TNF信号传导和T细胞受体信号传导。通过将我们的数据与其他细胞系中严重急性呼吸综合征冠状病毒2感染的已发表转录组进行比较，猴痘和COVID-19的共同功能包括免疫系统中的细胞因子信号传导、TNF信号传导和MAPK级联调节。因此，我们的数据表明，在COVID-19和猴痘之间确定的分子联系阐明了猴痘的病因。
