Rauls D O, Ting S, Lund M
J Allergy Clin Immunol. 1986 May;77(5):673-6. doi: 10.1016/0091-6749(86)90407-0.
The enzymatic isotopic analysis of histamine requires extraction and concentration of [3H]-1-methylhistamine from the reaction mixture. The assay was modified to include enzymatic reaction at 0 degrees C to reduce blank values and direct application of protein-free reaction mixture to a thin-layer chromatography plate for isolating of the product. The analysis was quantitative down to 100 pg/ml of histamine, adequate for monitoring plasma histamine content.
组胺的酶促同位素分析需要从反应混合物中提取并浓缩[3H]-1-甲基组胺。该测定方法经过改进，包括在0℃下进行酶促反应以降低空白值，并将无蛋白反应混合物直接应用于薄层色谱板以分离产物。该分析对组胺的定量下限为100 pg/ml，足以监测血浆组胺含量。
