Liao Dan, Liu Xiaomei, He Limei, Yao Yuhong, Yuan Xiuying, Feng Poling, Li Cuifen, Liu Yanyan
Department of Gynaecology, SSL Central Hospital of Dongguan, Dongguan Third People's Hospital, Affiliated Dongguan Shilong People's.
Hospital of Southern Medical University, Dongguan, Guangdong, China.
Iran J Basic Med Sci. 2023 Apr;26(4):468-477. doi: 10.22038/IJBMS.2023.64845.14281.
Cervical cancer (CC) is the fourth most prevalent type of cancer in women worldwide and it is considered the leading cause of tumor-related death and malignancy. As part of complexes involved in epigenetic control, the proteins of the chromobox (CBX) family have been found to have a role in the growth of malignancies by preventing differentiation and increasing proliferation. Here, by a thorough investigation, we investigated the expression, prognostic significance, and immune infiltration of CBX in patients with CC.
Differential expression, clinicopathological parameters, immune cell infiltration, enrichment analysis, genetic alteration, and prognostic value of CBXs in patients with CC were examined using TIMER, Metascape, STRING, GeneMANIA, cBioPortal, UALCAN, The Human Protein Atlas, Gene Expression Profiling Interactive Analysis (GEPIA), and Oncomine.
In CC tissues, CBX 2/3/4/5 and CBX 8 expression levels were considerably higher, whereas CBX 6/7 expression levels were lower. In CC, the CBX 5/6/8 promoters have elevated levels of methylation. The expression of CBX 2/6/8 and the pathological stage were connected. A 37% mutation rate of the differentially expressed CBX genes was observed. Also, there was a strong correlation of the CBXs expression with immune cell infiltration, such as T CD4 cells, macrophages, neutrophils, B cells, T CD8 cells, and dendritic cells.
The investigation discovered that members of the CBXs family may be therapeutic targets for CC patients and may play significant roles in the development of CC tumors.
宫颈癌（CC）是全球女性中第四大常见癌症类型，被认为是肿瘤相关死亡和恶性肿瘤的主要原因。作为参与表观遗传控制的复合物的一部分，已发现染色体盒（CBX）家族的蛋白质通过阻止分化和增加增殖在恶性肿瘤生长中发挥作用。在此，通过全面调查，我们研究了CC患者中CBX的表达、预后意义和免疫浸润情况。
使用TIMER、Metascape、STRING、GeneMANIA、cBioPortal、UALCAN、人类蛋白质图谱、基因表达谱交互分析（GEPIA）和Oncomine检测CC患者中CBXs的差异表达、临床病理参数、免疫细胞浸润、富集分析、基因改变和预后价值。
在CC组织中，CBX 2/3/4/5和CBX 8的表达水平显著更高，而CBX 6/7的表达水平较低。在CC中，CBX 5/6/8启动子的甲基化水平升高。CBX 2/6/8的表达与病理分期相关。观察到差异表达的CBX基因的突变率为37%。此外，CBXs的表达与免疫细胞浸润，如T CD4细胞、巨噬细胞、中性粒细胞、B细胞、T CD8细胞和树突状细胞，有很强的相关性。
该研究发现CBXs家族成员可能是CC患者的治疗靶点，并且可能在CC肿瘤的发生发展中起重要作用。
