Bandla Sai Suresh, Bhatt Rushil, Devadiga Santhosha
Father Muller Research Centre, Father Muller Medical College, Kankanady, Mangalore, 575002 Karnataka India.
Independent Researcher, Hyderabad, India.
Virusdisease. 2023 Mar;34(1):1-14. doi: 10.1007/s13337-022-00802-x. Epub 2022 Nov 27.
Respiratory syncytial virus (RSV) is known to be the major cause of lower respiratory tract infections in infants and in the elderly. RSV was recently reclassified and simplified into three genotypes of the RSV-A subgroup (GA1-GA3) and into seven genotypes of the RSV-B subgroup (GB1-GB7). This classification strategy was not implemented globally. This study intended to reclassify the sequences that were submitted in GenBank till September 2021 from India. The gene sequences of the ectodomain region, second hypervariable region (SHR), and the partial second hypervariable region (PSHR) of the G gene were selected for the analysis. 25 ectodomain, 36 s hypervariable, and 19 partial second hypervariable regions of the RSV-A subgroup and 42-ectodomain, 49-s hypervariable region and 11-partial second hypervariable region of RSV-B subgroup were used for phylogenetic analysis. P-distance was calculated to support the genotype determination done by phylogenetic analysis. Phylogenetic analysis revealed that GA2.3.1, GA2.3.3, GA2.3.4, GA2.3.5, and GA2.3.6b lineages of GA2 genotype for RSV-A; and GB5.0.1, GB5.0.2, GB5.0.3, GB5.0.4a, GB5.0.4c, GB5.0.5a, GB5.0.5c lineages of GB5 genotype and GB7 genotype for RSV-B were that circulated in India. This work has implication for RSV vaccine research, and also for strategies for the prevention and control of RSV infection in humans.
The online version contains supplementary material available at 10.1007/s13337-022-00802-x.
呼吸道合胞病毒（RSV）是已知的婴儿和老年人下呼吸道感染的主要原因。RSV最近被重新分类并简化为RSV - A亚组的三种基因型（GA1 - GA3）和RSV - B亚组的七种基因型（GB1 - GB7）。这种分类策略并未在全球范围内实施。本研究旨在对截至2021年9月从印度提交到GenBank的序列进行重新分类。选择G基因的胞外域区域、第二高变区（SHR）和部分第二高变区（PSHR）的基因序列进行分析。RSV - A亚组的25个胞外域、36个高变区和19个部分第二高变区以及RSV - B亚组的42个胞外域、49个高变区和11个部分第二高变区用于系统发育分析。计算P距离以支持系统发育分析所做的基因型确定。系统发育分析显示，RSV - A的GA2基因型的GA2.3.1、GA2.3.3、GA2.3.4、GA2.3.5和GA2.3.6b谱系；以及RSV - B的GB5基因型的GB5.0.1、GB5.0.2、GB5.0.3、GB5.0.4a、GB5.0.4c、GB5.0.5a、GB5.0.5c谱系和GB7基因型在印度流行。这项工作对RSV疫苗研究以及人类RSV感染的预防和控制策略具有重要意义。
在线版本包含可在10.1007/s13337 - 022 - 00802 - x获取的补充材料。
