Division of Clinical Basis for ES Cell Research, Center for Human ES Cell Research, Institute for Life and Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Emeritus Professor, Kyoto University, Kyoto 606-8351, Japan.
Biomater Sci. 2023 May 2;11(9):2974-2987. doi: 10.1039/d2bm01473d.
Although human pluripotent stem cell (hPSC) lines were initially established in culture using feeder cells, the development of culture media and substrates is essential for safe, stable, high-quality, and efficient production of large numbers of cells. Many researchers are now culturing hPSCs in chemically defined media and on culture substrates without feeder cells. In this review, we first discuss the problems with Matrigel, which has long been used as a culture substrate. Then, we summarize the development of extracellular matrix proteins for hPSCs, which are now the mainstream alternative, and synthetic substrates that are expected to be the future mainstream alternative. We also highlight three-dimensional culture for suitable mass production of hPSCs.
虽然人类多能干细胞（hPSC）系最初是在饲养细胞存在的情况下在培养中建立的，但培养基和底物的开发对于安全、稳定、高质量和高效率地大量生产细胞是至关重要的。许多研究人员现在正在无饲养细胞的情况下在化学成分确定的培养基中和在培养底物上培养 hPSC。在这篇综述中，我们首先讨论了长期以来一直被用作培养底物的 Matrigel 存在的问题。然后，我们总结了用于 hPSC 的细胞外基质蛋白的发展，这些蛋白现在是主流的替代物，以及有望成为未来主流替代物的合成底物。我们还强调了适合 hPSC 大规模生产的三维培养。
