Yu Jieyao, Ho Chi-Tang, Lin Zhi, Zhu Yin, Feng Zhihui, Ni Dejiang, Zeng Shengchun, Zeng Xuehong, Wang Yijun, Ning Jingming, Zhang Liang, Zhai Xiaoting, Wan Xiaochun
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China.
International Joint Laboratory on Tea Chemistry and Health Effects of Ministry of Education, Anhui Agricultural University, Hefei 230036, China.
J Agric Food Chem. 2023 Apr 3. doi: 10.1021/acs.jafc.3c00486.
The volatile fraction was isolated from the premium and common grade of green tea infusion by solvent-assisted flavor evaporation distillation. With the application of aroma extract dilution analysis, a total of 52 aroma-active compounds were revealed in the flavor dilution (FD) factor area of 32-8192. Besides, five additional odorants with higher volatility were identified using solid-phase microextraction. The aroma profiles, FD factors, and quantitative data of premium (PGP) and common (CGP) showed apparent differences. The intensity of the flowery attribute was significantly higher in PGP than in CGP, while cooked vegetable-like was the most outstanding odor note in CGP. The recombination experiment and the omission test of PGP clarified that dimethyl sulfide, (,)-2,4-heptadienal, ()-β-ionone, (,)-2,6-nonadienal, 2-methylbutanal, indole, 6-methyl-5-hepten-2-one, hexanal, 3-methylbutanal, γ-hexalactone, methyl epijasmonate, linalool, geraniol, and ()-3-hexen-1-ol were the key odorants of PGP tea infusion. The omission and addition tests of flowery odorants manifested that ()-β-ionone, geraniol, and (,)-2,4-heptadienal, with higher odor activity values in PGP than in CGP, contributed to the flowery attribute most. The difference in the concentration of the abovementioned odorants with flowery odor notes could be one of the main factors which led to the difference in aroma quality between the two grades of .
通过溶剂辅助风味蒸发蒸馏从优质和普通等级的绿茶浸出液中分离出挥发性成分。应用香气提取物稀释分析,在风味稀释(FD)因子为32 - 8192的区域共鉴定出52种香气活性化合物。此外,使用固相微萃取鉴定出另外5种挥发性更高的气味物质。优质绿茶浸出液(PGP)和普通绿茶浸出液(CGP)的香气特征、FD因子和定量数据存在明显差异。PGP中花香属性的强度显著高于CGP,而类似煮熟蔬菜的气味是CGP中最突出的气味特征。PGP的重组实验和缺失试验表明,二甲基硫醚、(E,E)-2,4-庚二烯醛、(E)-β-紫罗兰酮、(E,E)-2,6-壬二烯醛、2-甲基丁醛、吲哚、6-甲基-5-庚烯-2-酮、己醛、3-甲基丁醛、γ-己内酯、甲基表茉莉酸酯、芳樟醇、香叶醇和(E)-3-己烯-1-醇是PGP茶浸出液的关键气味物质。花香气味物质的缺失和添加试验表明,PGP中气味活性值高于CGP的(E)-β-紫罗兰酮、香叶醇和(E,E)-2,4-庚二烯醛对花香属性贡献最大。上述具有花香气味的气味物质浓度差异可能是导致这两个等级绿茶香气品质差异的主要因素之一。