体外形成骨的同步放射自显影和组织化学分析

Simultaneous autoradiographic and histochemical analysis of bone formed in vitro.

作者信息

Tenenbaum H C, McCulloch C A, Palangio K

出版信息

J Histochem Cytochem. 1986 Jun;34(6):769-73. doi: 10.1177/34.6.3701035.

DOI:10.1177/34.6.3701035

Abstract

The simultaneous histochemical demonstration of alkaline phosphatase activity and autoradiographic demonstration of [3H]-thymidine uptake is valuable for study of bone cell kinetics in vivo or in vitro. By use of this technique, it has been possible to detect changes induced by a single dose of dexamethasone (10(-7) M) in the time course of alkaline phosphatase activity, the number of alkaline phosphatase-positive cells, and [3H]-thymidine labeling in bone formed in vitro.

摘要

碱性磷酸酶活性的同时组织化学显示和[3H] - 胸腺嘧啶核苷摄取的放射自显影显示，对于体内或体外骨细胞动力学的研究很有价值。通过使用这种技术，已经能够检测单剂量地塞米松（10^(-7) M）在体外形成的骨中碱性磷酸酶活性的时间进程、碱性磷酸酶阳性细胞数量以及[3H] - 胸腺嘧啶核苷标记所诱导的变化。