一种用于检测杂交瘤细胞上清液中抗独特型的硝酸纤维素膜斑点筛选方法。
A dot screening procedure on nitrocellulose for detecting anti-idiotopes in hybridoma supernatants.
作者信息
Borden P, Kabat E A
出版信息
J Immunol Methods. 1986 May 22;89(2):229-31. doi: 10.1016/0022-1759(86)90362-5.
PMID:3701074
Abstract
A dot assay on nitrocellulose was developed for screening hybridoma fusions for the presence of anti-idiotopes. The technique involves spotting of hybridoma supernatants onto nitrocellulose filters, and detection of anti-idiotope with an alkaline phosphatase-coupled idiotype.
摘要
为筛选存在抗独特型的杂交瘤融合体，开发了一种用于硝酸纤维素膜的斑点试验。该技术包括将杂交瘤上清液点样到硝酸纤维素滤膜上，并用碱性磷酸酶偶联的独特型检测抗独特型。
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