Kusuzaki K, Kuzuhara A, Takeshita H, Ban S, Yamashita F, Sakakida K, Kamachi M, Ashihara T
Nihon Seikeigeka Gakkai Zasshi. 1986 Jan;60(1):51-60.
This study was undertaken to clarify the relationship between the proliferative activity and histological findings of the giant cell tumor (GCT) of bone by means of an epi-illumination cytofluorometer (NIKON SPM-RF1-D). Fresh tissues of GCT were surgically obtained from two cases. In both cases, small pieces of tumor tissues were obtained from several different regions based on the macroscopic characteristics of the cut surface, and processed for single cell preparation using enzymatic method. These isolated cells were smeared and stained with acridine orange, and then analyzed cytofluorometrically to determine simultaneously DNA and RNA contents of the individual cells. The results showed that the proliferative activity of tumor cells was much higher in the regions composed of both many histiocytic stromal cells having polygonal or ovoid shape and many multinucleated giant cells, than either in the regions composed of fibrocytic stromal cells accompanying abundant collagen fibers or in the regions composed of foamy cells.
本研究旨在通过落射式细胞荧光计（尼康SPM-RF1-D）阐明骨巨细胞瘤（GCT）的增殖活性与组织学表现之间的关系。从2例患者手术获取GCT新鲜组织。在这2例中，根据切面的宏观特征从几个不同区域获取小块肿瘤组织，并用酶法处理以制备单细胞。将这些分离的细胞涂片并用吖啶橙染色，然后进行细胞荧光分析以同时测定单个细胞的DNA和RNA含量。结果显示，在由许多具有多边形或卵圆形的组织细胞性基质细胞和许多多核巨细胞组成的区域中，肿瘤细胞的增殖活性比由伴有大量胶原纤维的纤维细胞性基质细胞组成的区域或由泡沫细胞组成的区域高得多。
