杂草黑麦（Secale cereale ssp. segetale）叶绿体基因组全序列的分子结构、比较和系统发育分析。
Molecular structure, comparative and phylogenetic analysis of the complete chloroplast genome sequences of weedy rye Secale cereale ssp. segetale.
机构信息
Institute of Biology, University of Szczecin, 71415, Szczecin, Poland.
Centre for Molecular Biology and Biotechnology, Institute of Biology, University of Szczecin, 71415, Szczecin, Poland.
出版信息
Sci Rep. 2023 Apr 3;13(1):5412. doi: 10.1038/s41598-023-32587-4.
The complete chloroplast genome of Secale cereale ssp. segetale (Zhuk.) Roshev. (Poaceae: Triticeae) was sequenced and analyzed to better use its genetic resources to enrich rye and wheat breeding. The study was carried out using the following methods: DNA extraction, sequencing, assembly and annotation, comparison with other complete chloroplast genomes of the five Secale species, and multigene phylogeny. As a result of the study, it was determined that the chloroplast genome is 137,042 base pair (bp) long and contains 137 genes, including 113 unique genes and 24 genes which are duplicated in the IRs. Moreover, a total of 29 SSRs were detected in the Secale cereale ssp. segetale chloroplast genome. The phylogenetic analysis showed that Secale cereale ssp. segetale appeared to share the highest degree of similarity with S. cereale and S. strictum. Intraspecific diversity has been observed between the published chloroplast genome sequences of S. cereale ssp. segetale. The genome can be accessed on GenBank with the accession number (OL688773).
黑麦亚种的完整叶绿体基因组（Zhuk.）Roshev.（禾本科：小麦族）被测序和分析，以更好地利用其遗传资源丰富黑麦和小麦的育种。该研究采用以下方法进行：DNA 提取、测序、组装和注释，与其他五个黑麦属物种的完整叶绿体基因组进行比较，以及多基因系统发育分析。研究结果表明，叶绿体基因组长 137042 个碱基对（bp），包含 137 个基因，其中 113 个为特有基因，24 个基因在 IRs 中重复。此外，在黑麦亚种的叶绿体基因组中检测到了 29 个 SSRs。系统发育分析表明，黑麦亚种与黑麦和密穗山羊草具有最高的相似度。在已发表的黑麦亚种的叶绿体基因组序列中观察到了种内多样性。该基因组可在 GenBank 上以注册号（OL688773）访问。
Zotero 插件