Aşır F, Özgökce Ç
Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey.
Eur Rev Med Pharmacol Sci. 2023 Mar;27(6):2182-2188. doi: 10.26355/eurrev_202303_31751.
We investigate the effect of Momordica charantia (MC) against ovarian ischemia-reperfusion injury (IRI).
Forty-eight Sprague Dawley female rats were divided into 6 groups. 3 hours ischemia/3 hours reperfusion was conducted. Before and/or after IR, 600 mg/kg MC were introduced to rats via an orogastric tube. At the end of the experiment, total serum antioxidant/oxidant status (TAS/TOS) and Anti-Mullerian Hormone (AMH) level were measured. Ovarian histopathology and APAF-1 expression level were analyzed.
TAS and AMH levels were the lowest, while TOS level and OSI were the highest in the IR group. TAS and AMH levels were higher, and TOS levels and OSI were lower in MC-treated groups compared to IR group. Follicular degeneration, granulosa and stromal cell degeneration, mononuclear cell infiltration and vascular congestion and dilatation were observed in the IR group. Ovarian histopathology was improved in groups that received MC extract. APAF-1 immune activity was intense in IR and MC+IR groups while decreased in the groups treated with MC extract after IRI. MC treatment downregulated APAF-1 protein after IRI.
MC extract restored negative biochemical and histochemical changes caused by IRI due to its antioxidant properties and supported cell survival by suppressing APAF-1 expression.
我们研究苦瓜（MC）对卵巢缺血再灌注损伤（IRI）的影响。
将48只雌性Sprague Dawley大鼠分为6组。进行3小时缺血/3小时再灌注。在缺血再灌注之前和/或之后，通过灌胃管给大鼠注入600mg/kg苦瓜。实验结束时，测量血清总抗氧化/氧化状态（TAS/TOS）和抗苗勒管激素（AMH）水平。分析卵巢组织病理学和凋亡蛋白酶激活因子1（APAF-1）表达水平。
在缺血再灌注组中，TAS和AMH水平最低，而TOS水平和氧化应激指数（OSI）最高。与缺血再灌注组相比，苦瓜处理组的TAS和AMH水平较高，TOS水平和OSI较低。在缺血再灌注组中观察到卵泡变性、颗粒细胞和基质细胞变性、单核细胞浸润以及血管充血和扩张。接受苦瓜提取物的组卵巢组织病理学得到改善。在缺血再灌注组和苦瓜+缺血再灌注组中，APAF-1免疫活性较强，而在缺血再灌注后用苦瓜提取物处理的组中APAF-1免疫活性降低。缺血再灌注后苦瓜处理下调了APAF-1蛋白。
苦瓜提取物因其抗氧化特性恢复了缺血再灌注引起的负面生化和组织化学变化，并通过抑制APAF-1表达支持细胞存活。
