OCT1 通过作用于 LDHA 来调节结直肠癌细胞的迁移。

OCT1 regulates the migration of colorectal cancer cells by acting on LDHA.

机构信息

Editorial Office, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Henan, PR China.

Department of Gastrointestinal Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Henan, PR China.

出版信息

Histol Histopathol. 2024 Jan;39(1):67-77. doi: 10.14670/HH-18-613. Epub 2023 Mar 23.

DOI:10.14670/HH-18-613

PMID:37014018

Abstract

Colorectal cancer is one of the most common cancers with high morbidity and mortality. Effective treatments to improve the prognosis are still lacking. The results of online analysis tools showed that OCT1 and LDHA were highly expressed in colorectal cancer, and the high expression of OCT1 was associated with poor prognosis. Immunofluorescence demonstrated that OCT1 and LDHA co-localized in colorectal cancer cells. In colorectal cancer cells, OCT1 and LDHA were upregulated by OCT1 overexpression, but downregulated by OCT1 knockdown. OCT1 overexpression promoted cell migration. OCT1 or LDHA knockdown inhibited the migration, and the downregulation of LDHA restored the promoting effect of OCT1 overexpression. OCT1 upregulation increased the levels of HK2, GLUT1 and LDHA proteins in colorectal cancer cells. Consequently, OCT1 promoted the migration of colorectal cancer cells by upregulating LDHA.

摘要

结直肠癌是最常见的癌症之一，具有较高的发病率和死亡率。目前仍缺乏有效的治疗方法来改善预后。在线分析工具的结果表明，OCT1 和 LDHA 在结直肠癌中高表达，且 OCT1 的高表达与不良预后相关。免疫荧光实验表明，OCT1 和 LDHA 在结直肠癌细胞中共定位。在结直肠癌细胞中，OCT1 过表达上调 OCT1 和 LDHA 的表达，而 OCT1 敲低则下调 OCT1 和 LDHA 的表达。OCT1 过表达促进细胞迁移。OCT1 或 LDHA 敲低抑制迁移，下调 LDHA 恢复 OCT1 过表达的促进作用。OCT1 上调增加了结直肠癌细胞中 HK2、GLUT1 和 LDHA 蛋白的水平。因此，OCT1 通过上调 LDHA 促进结直肠癌细胞的迁移。