University North, Department of Food Technology, Koprivnica, Croatia.
University North, Department for Packaging, Recycling and Environmental Protection, Koprivnica, Croatia.
Arh Hig Rada Toksikol. 2023 Apr 4;74(1):8-15. doi: 10.2478/aiht-2023-74-3693. Print 2023 Mar 1.
Microfiltration is a common step in liquid chromatography - tandem mass spectrometry (LC-MS), a method of choice in determining several mycotoxins in a solution at once. However, microfiltration may entail filter-analyte interactions that can affect the accuracy of the procedure, and underestimate exposure. The aim of our study was to assess how five different membrane materials for syringe filters (nylon, polytetrafluoroethylene, polyethersulphone, mixed cellulose ester, and cellulose acetate) affect microfiltration and recovery of EU-regulated mycotoxins, including aflatoxins B1, B2, G1, and G2, deoxynivalenol, fumonisins B1 and B2, zearalenone, T-2 and HT-2 toxins, and ochratoxin A. Polytetrafluoroethylene filters turned out to least affect microfiltration through mycotoxin loss, followed by more commonly used nylon filters, whereas the remaining three filter membrane materials had such a negative effect on recoveries that we found them incompatible with the procedure. Our findings clearly suggest that it is important to select a proper filter type that suits analyte properties and solution composition and to discard the first few filtrate drops to ensure the accuracy of the analytical procedure.
微孔过滤是液相色谱-串联质谱（LC-MS）的常见步骤，是同时测定溶液中几种霉菌毒素的首选方法。然而，微孔过滤可能需要进行滤膜-分析物相互作用，这可能会影响该过程的准确性，并低估暴露量。我们的研究旨在评估五种不同的注射器过滤器膜材料（尼龙、聚四氟乙烯、聚醚砜、混合纤维素酯和醋酸纤维素）如何影响欧盟规定的霉菌毒素（包括黄曲霉毒素 B1、B2、G1 和 G2、脱氧雪腐镰刀菌烯醇、伏马菌素 B1 和 B2、玉米赤霉烯酮、T-2 和 HT-2 毒素以及赭曲霉毒素 A）的微孔过滤和回收率。结果表明，聚四氟乙烯滤器对霉菌毒素损失的微孔过滤影响最小，其次是更常用的尼龙滤器，而其余三种滤膜材料对回收率的影响如此之大，以至于我们发现它们与该过程不兼容。我们的研究结果清楚地表明，选择适合分析物性质和溶液组成的适当滤器类型并丢弃最初的几毫升滤液以确保分析程序的准确性非常重要。
