从恢复系 R186 中鉴定出棉花细胞质雄性不育育性恢复候选基因。

Identification of fertility restoration candidate genes from a restorer line R186 for Gossypium harknessii cytoplasmic male sterile cotton.

机构信息

Laboratory of Cotton Genetics, Genomics and Breeding /Key Laboratory of Crop Heterosis and Utilization of Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Haidian District, No. 2, Yuanmingyuan West Rd, Beijing, 100193, China.

出版信息

BMC Plant Biol. 2023 Apr 4;23(1):175. doi: 10.1186/s12870-023-04185-z.

DOI:10.1186/s12870-023-04185-z

PMID:37016285

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10071737/

Abstract

BACKGROUND

The utilization of heterosis based on three-line system is an effective strategy in crop breeding. However, cloning and mechanism elucidation of restorer genes for cytoplasmic male sterility (CMS) in upland cotton have yet been realized.

RESULTS

This research is based on CMS line 2074A with the cytoplasm from Gossypium harknessii (D) and restorer line R186. The offspring of 2074A × R186 were used to conduct genetic analysis. The fertility mechanism of 2074A can be speculated to be governed by multiple genes, since neither the single gene model nor the double genes model could be used. The bulked segregant analysis (BSA) for (2074A × R186) F determined the genetic interval of restorer genes on a region of 4.30 Mb on chromosome D05 that contains 77 annotated genes. Four genes were identified as candidates for fertility restoration using the RNA-seq data of 2074A, 2074B, and R186. There are a number of large effect variants in the four genes between 2074A and R186 that could cause amino acid changes. Evolutionary analysis and identity analysis revealed that GH_D05G3183, GH_D05G3384, and GH_D05G3490 have high identity with their homologs in D, respectively. Tissue differential expression analysis revealed that the genes GH_D05G3183, GH_D05G3384, and GH_D05G3490 were highly expressed in the buds of the line R186. The predicted results demonstrated that GH_D05G3183, GH_D05G3384 and GH_D05G3490 might interact with GH_A02G1295 to regulate orf610a in mitochondria.

CONCLUSION

Our study uncovered candidate genes for fertility restoration in the restorer line R186 and predicted the possible mechanism for restoring the male fertility in 2074A. This research provided valuable insight into the nucleoplasmic interactions.

摘要

背景

基于三系法杂种优势的利用是作物育种的有效策略。然而，陆地棉细胞质雄性不育（CMS）恢复基因的克隆和机制阐明尚未实现。

结果

本研究以细胞质来自海岛棉（D）的 CMS 系 2074A 和恢复系 R186 为基础。利用 2074A×R186 的后代进行遗传分析。推测 2074A 的育性机制受多个基因控制，因为既不能用单基因模型也不能用双基因模型来解释。对（2074A×R186）F 的混池分离分析（BSA）确定了在包含 77 个注释基因的 D05 染色体上的恢复基因遗传区间为 4.30 Mb。利用 2074A、2074B 和 R186 的 RNA-seq 数据，鉴定出 4 个与育性恢复相关的候选基因。在 2074A 和 R186 之间，这四个基因中有许多大效应变异，可能导致氨基酸变化。进化分析和同一性分析表明，GH_D05G3183、GH_D05G3384 和 GH_D05G3490 分别与 D 中的同源物具有高度的同一性。组织差异表达分析表明，基因 GH_D05G3183、GH_D05G3384 和 GH_D05G3490 在 R186 的芽中高度表达。预测结果表明，GH_D05G3183、GH_D05G3384 和 GH_D05G3490 可能与 GH_A02G1295 相互作用，以调节线粒体中的 orf610a。