MD, MSc, PhDc, Lecturer/Medical Biologist, Laboratory of Hematology, University Joseph KiZerbo, 01 BP 7021 Ouagadougou 01, Burkina Faso.
MD, MSc, PhDc, Lecturer/Medical Biologist, Laboratory of Hematology, University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso.
Immunohematology. 2023 Apr 5;39(1):35-42. doi: 10.21307/immunohematology-2023-007. Print 2023 Apr 1.
To date, 43 blood group systems with 349 red blood cell (RBC) antigens have been recognized. The study of their distribution is useful for blood services to improve their supply strategies for providing blood of rare phenotypes, but also to design indigenous RBC panels for alloantibody screening and identification. In Burkina Faso, the distribution of extended blood group antigens is not known. This study aimed to investigate the extended profiles of blood group antigens and phenotypes of this population and to raise limitations and potential strategies for the design of local RBC panels. We conducted a cross-sectional study that included group O blood donors. Extended phenotyping for antigens in the Rh, Kell, Kidd, Duffy, Lewis, MNS, and P1PK systems was performed using the conventional serologic tube technique. The prevalence of each antigen and phenotype combination was determined. A total of 763 blood donors were included. The majority were positive for D, c, e, and k and negative for Fy and Fy. The prevalence of K, Fy, Fy, and C was less than 5 percent. The most frequent Rh phenotype was Dce, and the most common probable haplotype was RR (69.5%). For the other blood group systems, the K-k+ (99.4%), M+N+S+s- (43.4%), and Fy(a-b-) (98.8%) phenotypes were the most frequent. Antigenic polymorphism of blood group systems by ethnicity and geography argues for the design and evaluation of population-sourced RBC panels to meet specific antibody profiles. However, some of the specificities identified in our study, such as the rarity of double-dose antigen profiles for certain antigens and the cost of antigen phenotyping tests, are major challenges to overcome.
迄今为止，已经发现了 43 个血型系统和 349 种红细胞（RBC）抗原。研究它们的分布对于血液服务机构改善稀有表型血液的供应策略非常有用，但也可以设计用于同种异体抗体筛选和鉴定的本土 RBC 面板。在布基纳法索，尚未了解扩展血型抗原的分布情况。本研究旨在调查该人群的扩展血型抗原和表型，并提出设计本地 RBC 面板的局限性和潜在策略。我们进行了一项横断面研究，包括 O 型血献血者。使用传统的血清学试管技术对 Rh、Kell、Kidd、Duffy、Lewis、MNS 和 P1PK 系统中的抗原进行了扩展表型分析。确定了每个抗原和表型组合的流行率。共有 763 名献血者入组。大多数人对 D、c、e 和 k 呈阳性，对 Fy 和 Fy 呈阴性。K、Fy、Fy 和 C 的流行率低于 5%。最常见的 Rh 表型是 Dce，最常见的可能单倍型是 RR（69.5%）。对于其他血型系统，K-k+（99.4%）、M+N+S+s-（43.4%）和 Fy(a-b-)（98.8%）表型最为常见。血型系统的抗原多态性因种族和地理位置而异，这就需要设计和评估基于人群的 RBC 面板，以满足特定的抗体谱。然而，我们在研究中发现的一些特异性，如某些抗原的双剂量抗原谱的稀有性以及抗原表型检测的成本，是需要克服的主要挑战。
