Baumeister W, Barth M, Hegerl R, Guckenberger R, Hahn M, Saxton W O
J Mol Biol. 1986 Jan 20;187(2):241-50. doi: 10.1016/0022-2836(86)90231-7.
The low-resolution structure of the regular surface layer of Deinococcus radiodurans has been determined from negatively stained specimens by three-dimensional electron microscopy. The layer has P6 symmetry, a lattice constant of 18 nm and a thickness of 6.5 nm. Three-dimensional reconstruction was performed by a hybrid real space/Fourier space approach that incorporates partial compensation of lattice distortions: The model obtained is discussed in the light of independent information about the surface structure of this layer, derived from metal shadowing and surface relief reconstruction. While agreement is quite satisfactory for the apparently more rigid inner surface, the outer surface shows severe flattening effects. The structure of the HPI layer is compared with other bacterial surface layers using a classification scheme that is outlined in the Appendix.
通过三维电子显微镜对经负染色的样本进行分析，确定了耐辐射球菌规则表面层的低分辨率结构。该层具有P6对称性，晶格常数为18纳米，厚度为6.5纳米。采用结合晶格畸变部分补偿的混合实空间/傅里叶空间方法进行三维重建：根据从金属阴影和表面起伏重建中获得的关于该层表面结构的独立信息，对得到的模型进行了讨论。虽然对于明显更刚性的内表面，一致性相当令人满意，但外表面显示出严重的扁平化效应。使用附录中概述的分类方案，将HPI层的结构与其他细菌表面层进行了比较。
