Oorschot D E, Jones D G
J Neurosci Res. 1986;15(3):341-52. doi: 10.1002/jnr.490150305.
A tissue culture model has been developed to examine the hypothesis that axons can only regenerate when their growing tips are surrounded by extracellular fluid containing proteins derived from the blood. Fetal rat cerebral explants were cultured in serum medium for 10 days, followed by serum-free (SF) medium (from which serum had been removed) until 18 days in vitro (DIV). All explants cultured in serum medium for 0-10 DIV exhibited greater than 77% neurite viability (neurite viability ratio, NVR, 3.10). This degree of neurite viability was maintained for those explants exposed to serum until 18 DIV (NVR 2.82 at 18 DIV). By contrast, explants maintained in SF medium from 10-18 DIV had a much lower NVR, which, by 18 DIV, had declined to 0.30 (7.5% viability). Transmission electron microscopic analysis of explants fixed at 18 DIV confirmed these phase-contrast results and also showed a predominance of axonal profiles within the neurite population. In the center of explants, tissue viability was in excess of 75% in both the serum and SF media, suggesting that serum is of primary importance for axonal extension rather than neuronal survival. These data strengthen the hypothesis that blood-derived proteins may be needed for prolonged regeneration.
已经建立了一种组织培养模型，以检验以下假设：只有当轴突的生长尖端被含有源自血液的蛋白质的细胞外液包围时，轴突才能再生。将胎鼠脑外植体在血清培养基中培养10天，然后在无血清（SF）培养基（已去除血清）中培养至体外18天（DIV）。在血清培养基中培养0至10 DIV的所有外植体均表现出大于77%的神经突活力（神经突活力比率，NVR，3.10）。对于那些暴露于血清直至18 DIV的外植体，这种神经突活力程度得以维持（18 DIV时NVR为2.82）。相比之下，在10至18 DIV期间维持在SF培养基中的外植体NVR要低得多，到18 DIV时已降至0.30（活力为7.5%）。对18 DIV固定的外植体进行的透射电子显微镜分析证实了这些相差显微镜观察结果，并且还显示神经突群体中轴突轮廓占优势。在外植体中心，血清和SF培养基中的组织活力均超过75%，这表明血清对轴突延伸而非神经元存活至关重要。这些数据强化了以下假设：延长再生可能需要源自血液的蛋白质。
