Marghich Mohamed, Amrani Ouafa, Karim Ahmed, Harit Tarik, Beyi Leila, Mekhfi Hassane, Bnouham Mohamed, Aziz Mohammed
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, Mohammed First University, 60000 Oujda, Morocco.
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Department of Biology, Faculty of Sciences, Mohammed First University, 60000 Oujda, Morocco.
J Ethnopharmacol. 2023 Jul 15;311:116456. doi: 10.1016/j.jep.2023.116456. Epub 2023 Apr 3.
Gastrointestinal disorders are among the most common diseases that cause discomfort to people who are affected. In Morocco, aromatic and medicinal plants are widely used to calm these pains and eliminate their symptoms. Among these plants, Artemisia campestris L. which is used in eastern Morocco to treat digestive system problems.
Our study aimed to experimentally verify the traditional use of this plant by evaluating the myorelaxant and antispasmodic effects of the essential oil of Artemisia campestris L. (EOAc).
Gas Chromatography-Mass Spectrometry analysis (GC-MS) was performed to identify the compounds present in the EOAc. Then, these molecules were subjected to the in silico study for molecular docking. The myorelaxant and antispasmodic evaluation of the EOAc were tested in vitro on an isolated rabbit and rat jejunum mounted on an organ bath. Then, an isotonic transducer connected to an amplifier recorded the graph related to intestinal contractility.
GC-MS analysis of the essential oil of Artemisia campestris L. showed the presence of m-Cymene (17.308%), Spathulenol (16.785%), β Pinene (15.623%), α Pinene (11.352%), α.-Campholenal (8.848%) as main constituents. The EOAc gave a dose-dependent and reversible myorelaxant effect on the spontaneous contractions of jejunum isolated from rabbits, with an IC equal to 72.16 ± 15.93 μg/mL. This effect did not occur through adrenergic receptors. The EOAc has an antispasmodic effect on the contractions of rat jejunal induced by a medium with low (25 mM) or high concentration (75 mM) of KCl, and carbachol 10 M. The obtained inhibitory effects are comparable to those of a non-competitive antagonist of cholinergic receptors. The major compounds of EOAc allowed the establishment of a relationship between these phytoconstituents and the antispasmodic effect found by the EOAc. The obtained results are also supported by a docking study.
The obtained results confirm favorably the use of Artemisia campestris L. in traditional Moroccan medicine for the treatment of digestive tract illness, which gives us a new route to valorize the effects obtained by a phytomedicine specific for the digestive tract.
胃肠道疾病是最常见的使人不适的疾病之一。在摩洛哥，芳香植物和药用植物被广泛用于缓解这些疼痛并消除其症状。在这些植物中，摩洛哥东部使用山地蒿（Artemisia campestris L.）来治疗消化系统问题。
我们的研究旨在通过评估山地蒿精油（EOAc）的肌松作用和抗痉挛作用，以实验方式验证这种植物的传统用途。
采用气相色谱 - 质谱分析（GC - MS）来鉴定EOAc中存在的化合物。然后，对这些分子进行分子对接的计算机模拟研究。EOAc的肌松和抗痉挛评估在体外对安装在器官浴槽中的离体兔和大鼠空肠进行测试。然后，连接到放大器的等张换能器记录与肠道收缩性相关的图表。
山地蒿精油的GC - MS分析表明，主要成分有间 - 伞花烃（17.308%）、匙叶桉油烯醇（16.785%）、β - 蒎烯（15.623%）、α - 蒎烯（11.352%）、α - 樟烯醛（8.848%）。EOAc对离体兔空肠的自发收缩产生剂量依赖性和可逆的肌松作用，IC50等于72.16 ± 15.93μg/mL。这种作用不是通过肾上腺素能受体发生的。EOAc对低浓度（25mM）或高浓度（75mM）KCl以及10μM卡巴胆碱诱导的大鼠空肠收缩具有抗痉挛作用。所获得的抑制作用与胆碱能受体非竞争性拮抗剂的作用相当。EOAc的主要化合物使这些植物成分与EOAc发现的抗痉挛作用之间建立了联系。对接研究也支持了所获得的结果。
所获得的结果有力地证实了山地蒿在摩洛哥传统医学中用于治疗消化道疾病的用途，这为我们提供了一条新途径来评估一种针对消化道的植物药所获得的效果。
