Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; Chongqing School, University of Chinese Academy of Science, Chongqing 400714, China.
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; Chongqing School, University of Chinese Academy of Science, Chongqing 400714, China.
Int J Biol Macromol. 2023 Jun 1;239:124271. doi: 10.1016/j.ijbiomac.2023.124271. Epub 2023 Apr 3.
In this paper, the glass nanopore technology was proposed to detect a single molecule of starch dissolved in ionic liquid [1-butyl-3-methylimidazolium chloride (BmimCl)]. Firstly, the influence of BmimCl on nanopore detection is discussed. It is found that a certain amount of strong polar ionic liquids will disturb the charge distribution in nanopores and increase the detection noise. Then, by analysis of the characteristic current signal of the conical nanopore, the motion behaviour of starch near the entrance of the nanopore was studied and analysis the dominant ion of starch in the BmimCl dissolution process. Finally, based on nuclear magnetic resonance (NMR) and Fourier transform infrared (FTIR) spectroscopy simply discussed the mechanism of amylose and amylopectin dissolved in BmimCl. These results confirm that branched chain structure would affect the dissolution of polysaccharides in ionic liquids and the contribution of anions to the dissolution of polysaccharides are dominant. It is further proved that the current signal can be used to judge the charge and structure information of the analyte, and the dissolution mechanism can be assist analyzed at the single molecule level.
本文提出了玻璃纳米孔技术，用于检测溶解在离子液体[1-丁基-3-甲基咪唑氯（BmimCl）]中的单个淀粉分子。首先，讨论了 BmimCl 对纳米孔检测的影响。结果发现，一定量的强极性离子液体会干扰纳米孔中的电荷分布并增加检测噪声。然后，通过对锥形纳米孔特征电流信号的分析，研究了淀粉在纳米孔入口附近的运动行为，并分析了淀粉在 BmimCl 溶解过程中的主导离子。最后，基于核磁共振（NMR）和傅里叶变换红外（FTIR）光谱，简单讨论了直链淀粉和支链淀粉在 BmimCl 中的溶解机制。这些结果证实了支链结构会影响多糖在离子液体中的溶解，并且阴离子对多糖溶解的贡献是主导的。进一步证明了电流信号可用于判断分析物的电荷和结构信息，并可在单分子水平上辅助分析溶解机制。
