Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou, 310058, China.
Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
Environ Pollut. 2023 Jun 15;327:121557. doi: 10.1016/j.envpol.2023.121557. Epub 2023 Apr 3.
The partitioning and migration of antibiotic resistance genes (ARGs) at the interfaces of soil, water, and air play a critical role in the environmental transmission of antibiotic resistance. This study investigated the partitioning and migration of resistant plasmids as representatives of extracellular-ARGs (eARGs) in artificially constructed soil-water-air systems. Additionally, it quantitatively studied the influence of soil pH, clay mineral content, organic matter content, and simulated rainfall on the migration of eARGs via orthogonal experiments. The findings revealed that the sorption equilibrium between eARGs and soil can be attained within 3 h, following the two-compartment first-order kinetic model. The average partition ratio of eARGs in soil, water, and air is 7:2:1, and soil pH and clay mineral content are identified as the main influencing factors. The proportion of eARGs migrating from soil to water and air is 8.05% and 0.52%, respectively. Correlation and significance analyses showed that soil pH has a significant impact on the soil-water and soil-air mobility of eARGs, while clay content affects the percentage of peaks during migration. Moreover, rainfall exerts a noticeable impact on the timing of peaks during migration. This study provided quantitative insights into the proportion of eARGs in soil, water, and air and elucidated the key factors influencing the partitioning and migration of eARGs from the perspectives of the sorption mechanism.
抗生素抗性基因（ARGs）在土壤、水和空气界面的分配和迁移在抗生素抗性的环境传播中起着关键作用。本研究以抗性质粒作为细胞外抗生素抗性基因（eARGs）的代表，在人工构建的土壤-水-气系统中研究了它们的分配和迁移。此外，通过正交实验定量研究了土壤 pH 值、粘土矿物含量、有机质含量和模拟降雨对 eARGs 迁移的影响。结果表明，eARGs 与土壤之间的吸附平衡可以在 3 小时内达到，遵循双室一级动力学模型。eARGs 在土壤、水和空气中的分配比为 7:2:1，土壤 pH 值和粘土矿物含量是主要影响因素。eARGs 从土壤向水和空气迁移的比例分别为 8.05%和 0.52%。相关性和显著性分析表明，土壤 pH 值对 eARGs 的土壤-水和土壤-空气迁移有显著影响，而粘土含量影响迁移过程中的峰值比例。此外，降雨对迁移过程中峰值的时间有显著影响。本研究从吸附机制的角度提供了关于 eARGs 在土壤、水和空气中的比例以及影响 eARGs 分配和迁移的关键因素的定量见解。
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