Department of Biological Sciences and Biomolecular Sciences Institute, Florida International University, Miami, Florida 33199, USA.
Department of Biological Sciences and Biomolecular Sciences Institute, Florida International University, Miami, Florida 33199, USA
Cold Spring Harb Protoc. 2023 Oct 3;2023(10):785-8. doi: 10.1101/pdb.prot108174.
The uniport olfactometer behavioral assay is currently one of the most reliable single-choice methods to use to study mosquito attraction to olfactory stimuli. It allows for the reproducible calculation of mosquito attraction rate to human hosts or to other olfactory stimuli. Here, we present the design of our modified uniport olfactometer. Consistent carbon-filtered air flows through the assay, creating positive pressure that reduces odor contamination from the room. It includes a precision-milled white acrylic base to facilitate easy setup and consistent placement of the component parts. Our design can be made by a commercial acrylic fabricator or an academic machine shop. This olfactometer is designed to assess the responses of mosquitoes but could be applied to other insects that fly upwind toward an odor stimulus. In a companion protocol, we detail how to perform the experiments with mosquitoes by using the uniport olfactometer.
单端口嗅觉仪行为测定法是目前研究蚊子对嗅觉刺激吸引力最可靠的单一选择方法之一。它可以重现计算蚊子对人类宿主或其他嗅觉刺激的吸引力。在这里，我们介绍我们改进的单端口嗅觉仪的设计。一致的碳过滤空气流过测定法，产生正压，减少来自房间的气味污染。它包括一个精密铣制的白色亚克力基座，以方便组件的轻松设置和一致放置。我们的设计可以由商业亚克力制造商或学术机械车间制造。该嗅觉仪旨在评估蚊子的反应，但也可以应用于其他飞向气味刺激的顺风飞行昆虫。在配套方案中，我们详细介绍了如何使用单端口嗅觉仪进行蚊子实验。
