Won Kyungho, Choi Eu Ddeum, Kim Keumsun, Jung Hae Won, Shin Il Sheob, Hong Seongsig, Segonzac Cécile, Kim Young Jin
Pear Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA), Naju 58216, Korea.
Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul 08826, Korea.
Plant Pathol J. 2023 Apr;39(2):228-233. doi: 10.5423/PPJ.NT.01.2023.0006. Epub 2023 Apr 1.
Two pear cultivars with different degrees of resistance to Venturia nashicola were evaluated on the basis of a disease severity rating for pear scab resistance under controlled environmental condition. Two inoculation techniques were tested: the procedure for inoculation by dropping conidia suspension of V. nashicola; the procedure by deposition of agar plug on the abaxial surface of pear leaves. All tested cultivars resulted in blight symptoms on the inoculated leaves and became spread to uninoculated region or other leaves. Although both methods provide satisfactory infection of V. nashicola on pear leaves, the mycelial plug method of inoculation was more reliable than the spray inoculation method for the evaluation of pear scab disease resistance. The incubation period of V. nashicola in the resistant pear cultivar, Greensis was longer than that in the susceptible cultivar, Hwasan.
在可控环境条件下,根据梨黑星病抗性的病情严重程度评级,对两个对梨黑星病菌(Venturia nashicola)具有不同抗性程度的梨品种进行了评估。测试了两种接种技术:滴加梨黑星病菌分生孢子悬浮液的接种程序;在梨叶背面放置琼脂块的程序。所有测试品种在接种叶片上均出现枯萎症状,并蔓延至未接种区域或其他叶片。虽然两种方法都能使梨黑星病菌在梨叶上获得满意的感染效果,但在评估梨黑星病抗性时,菌丝块接种法比喷雾接种法更可靠。梨黑星病菌在抗病梨品种“绿宝石”(Greensis)中的潜伏期比感病品种“华山”(Hwasan)中的更长。