Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Posta Veterinaria y Zootecnia de la Universidad Michoacana de San Nicolás de Hidalgo, km. 9.5 S/N carretera Morelia-Zinapécuaro, C.P. 58893, La Palma, Tarímbaro, Michoacán, México (Nuñez-Anita, Calderón-Rico, Pérez-Duran, Zamora-Avilés, Franco-Correa, Bravo-Patiño); Escuela Nacional de Estudios Superiores Unidad León, Universidad Nacional Autónoma de México, Blv. UNAM No. 2011, Predio el Saucillo, Comunidad los Tepetates, El Potrero, León, Guanajuato, México (Arenas-Arrocena, Hernandez-Morales).
Can J Vet Res. 2023 Apr;87(2):110-119.
The objective of this study was to analyze the response of lymphocytes from pigs naturally infected with porcine respiratory disease complex (PRDC) at 3 different stages of development. Porcine respiratory disease complexes were isolated from 2 groups: The infected group, consisting of pigs with PRDC and no vaccination against any virus ( = 24), and the control group, consisting of vaccinated and noninfected piglets ( = 24). Both groups were sampled at 3 stages of development: Weaning (WEA) ( = 8), initiation (INI) ( = 8), and growth (GRO) ( = 8). The PRDC status was confirmed by serological testing against porcine circovirus type 2 (PCV-2), porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (H1N1), and . PCV-2+ cells were quantified by flow cytometry. Weight gain was registered at each stage. PCV-2+ cells, CD4+ cells, monocytes and lymphocytes populations were measured. Gene expression in CD4+ cells was quantified for interferon-γ (IFN-γ), GATA binding protein 3 (GATA3), T-box transcription factor (T-bet), interleukin-10 (IL-10), and IL-4. Control piglets gained approximately 35% more weight than those infected with PRDC. Specifically, PCV-2+ cells were detected in piglets from the infected group in the following proportions: WEA ≤ INI ≤ GRO. In infected piglets, the CD4+ count increased at WEA and decreased at GRO, CD4+ expression profile showed an overexpression of T-bet at INI and GRO, and the expression of IFN-γ was lower at WEA and GRO. In contrast, IL-4 was overexpressed at all 3 stages. GATA3 was overexpressed at INI and GRO. The infected piglets showed lymphopenia and less CD4+ cells. CD4+ cells showed a different expression profile than the control group, in which IFN-γ was less expressed, whereas IL-4 and T-bet were overexpressed.
本研究旨在分析在猪呼吸疾病复合症（PRDC）发展的 3 个不同阶段自然感染的猪淋巴细胞的反应。从两组猪中分离出猪呼吸疾病复合症：感染组，由患有 PRDC 且未接种任何病毒的猪组成（=24 头），和对照组，由接种且未感染的仔猪组成（=24 头）。两组均在 3 个发育阶段采样：断奶（WEA）（=8）、启动（INI）（=8）和生长（GRO）（=8）。PRDC 状态通过针对猪圆环病毒 2 型（PCV-2）、猪繁殖与呼吸综合征病毒（PRRSV）、猪流感病毒（H1N1）和 的血清学检测来确认。通过流式细胞术定量 PCV-2+细胞。在每个阶段记录体重增加。测量 PCV-2+细胞、CD4+细胞、单核细胞和淋巴细胞群。定量 CD4+细胞中的干扰素-γ（IFN-γ）、GATA 结合蛋白 3（GATA3）、T 盒转录因子（T-bet）、白细胞介素-10（IL-10）和 IL-4 的基因表达。对照组仔猪比感染 PRDC 的仔猪体重增加约 35%。具体而言，在感染组的仔猪中检测到以下比例的 PCV-2+细胞：WEA≤INI≤GRO。在感染仔猪中，CD4+计数在 WEA 时增加，在 GRO 时减少，CD4+表达谱显示 INI 和 GRO 时 T-bet 过度表达，而在 WEA 和 GRO 时 IFN-γ表达水平较低。相反，IL-4 在所有 3 个阶段都过度表达。GATA3 在 INI 和 GRO 时过度表达。感染仔猪表现出淋巴细胞减少和 CD4+细胞减少。CD4+细胞表现出与对照组不同的表达谱，其中 IFN-γ表达水平较低，而 IL-4 和 T-bet 表达水平较高。
