Morrison A R, Pascoe N
Kidney Int. 1986 Feb;29(2):496-501. doi: 10.1038/ki.1986.27.
Administration of mercuric chloride (HgCl2, 6 mg/kg) to rabbits produced renal failure, with changes in serum creatinine from 1.01 +/- 1 in controls to 6.46 +/- 0.91 mg/dl 24 hr after administration. Mitochondria isolated from HgCl2-treated rabbits exhibited alterations in acceptor control ratios, with reduction to 1.9 +/- 0.2 from 3.9 +/- 1.2 in controls. Ultrastructurally, the mitochondria showed swelling and loss of inner mitochondrial membranes. Total lipids from mitochondria of control and treated rabbits were obtained by modified Folch extraction and phospholipids analyzed by TLC. Brush border membranes and basolateral membranes were prepared from control and HgCl2-treated kidneys 2 and 24 hr after HgC12 administration. At 24 hr mitochondria showed a 36% fall in phosphatidylcholine (PC), a 36% fall in phosphatidylethanolamine (PE), and a 27% fall in cardiolipin. Brush border showed a decrease in phosphatidylserine (PS) of 29% and in PE of 40%. The basolateral membranes showed a reduction only in PE of 35%. At 2 hr post HgCl2, early changes are confined to the BBM and consist of a reduction in PE in this membrane. This changes in membrane phospholipids may be important in the functional derangements that occur at the cellular level after HgCl2 administration.
给兔子注射氯化汞（HgCl2，6毫克/千克）会导致肾衰竭，给药后24小时血清肌酐从对照组的1.01±1升高至6.46±0.91毫克/分升。从经HgCl2处理的兔子中分离出的线粒体表现出受体控制率的改变，从对照组的3.9±1.2降至1.9±0.2。在超微结构上，线粒体显示肿胀和线粒体内膜丧失。通过改良的Folch提取法获得对照组和处理组兔子线粒体的总脂质，并用薄层色谱法分析磷脂。在给予HgC12后2小时和24小时，从对照组和经HgCl2处理的肾脏制备刷状缘膜和基底外侧膜。在24小时时，线粒体的磷脂酰胆碱（PC）下降36%，磷脂酰乙醇胺（PE）下降36%，心磷脂下降27%。刷状缘的磷脂酰丝氨酸（PS）下降29%，PE下降40%。基底外侧膜仅PE下降35%。在HgCl2给药后2小时，早期变化局限于刷状缘膜，表现为该膜中PE减少。HgCl2给药后细胞水平上发生的功能紊乱中，膜磷脂的这种变化可能很重要。
