Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Methods Mol Biol. 2023;2658:81-103. doi: 10.1007/978-1-0716-3155-3_7.
Genomic approaches are widely applied to study the genetic basis of antifungal drug resistance in clinical isolates and experimental studies. Whole-genome sequencing of clinical isolates can comprehensively identify mutations associated with drug resistance and their frequency across fungal populations. In addition, genome comparison of serially collected isolates, such as from patient samples or in vitro drug selection experiments, will identify a small number of changes that can be evaluated for association with drug resistance. Here, we provide a detailed protocol for the computational analysis of genome sequences to identify variants associated with drug resistance.
基因组学方法广泛应用于研究临床分离株中抗真菌药物耐药性的遗传基础和实验研究。临床分离株的全基因组测序可以全面鉴定与耐药性相关的突变及其在真菌群体中的频率。此外，对连续采集的分离株（如来自患者样本或体外药物选择实验）进行基因组比较，可以鉴定出少量可用于评估与耐药性相关的变化。在这里，我们提供了一个详细的基因组序列计算分析方案，以鉴定与耐药性相关的变异。
