Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, 83000, Mexico.
Universidade Estadual Paulista (UNESP), Sao Vicente, Sao Paulo, Brazil.
F1000Res. 2022 May 16;11:527. doi: 10.12688/f1000research.111181.2. eCollection 2022.
Several studies have shown that active compounds of (cardenolides) have antiproliferative effect on human cancer cells. Cardenolides isolated from can be used as active chemical markers to elaborate phytopharmaceutical preparations. The aim of this work was to evaluate the antiproliferative effect of a standardized extract of the aerial parts, based on cardenolides. Four standardized extracts were prepared by HPLC-DAD depending on the concentration of calotropin and the antiproliferative activity was measured for the MTT assay, on the A549, MCF-7, HeLa, PC3 and ARPE cell lines. The concentrations of calotropin used for the standardization of the extracts were 10, 7.6, 5 and 1 mg/dL. Standardization of the extract based on calotropin at 7.6 mg/g dry weight was achieved and the antiproliferative activity was evaluated over A549, HeLa and MCF-7 cell lines, obtaining proliferation percentages of 3.8 to 13.4% The standardized extracts of at different concentrations of calotropin showed antiproliferative activity against all the cell lines evaluated. The greatest effect was observed against the HeLa cell line.
几项研究表明，（强心苷）的活性化合物对人类癌细胞具有抗增殖作用。从羊角拗中分离出的强心苷可用作阐明植物药物制剂的活性化学标志物。本工作的目的是评估基于羊角拗强心苷的地上部分标准化提取物的抗增殖作用。根据 calotropin 的浓度和 MTT 测定的抗增殖活性，通过 HPLC-DAD 制备了四种标准化提取物，用于 A549、MCF-7、HeLa、PC3 和 ARPE 细胞系。用于提取物标准化的 calotropin 浓度为 10、7.6、5 和 1mg/dL。基于 7.6mg/g 干重 calotropin 的羊角拗提取物的标准化得以实现，并评估了其对 A549、HeLa 和 MCF-7 细胞系的抗增殖活性，获得了 3.8%至 13.4%的增殖百分比。不同浓度 calotropin 的羊角拗标准化提取物对所有评估的细胞系均表现出抗增殖活性。对 HeLa 细胞系的作用最大。
