中国仓鼠细胞中姐妹染色单体交换（SCEs）的诱导与突变之间的关系。I. 过氧化氢和咖啡因的实验。

The relationship between the induction of SCEs and mutations in Chinese hamster cells. I. Experiments with hydrogen peroxide and caffeine.

作者信息

Speit G

出版信息

Mutat Res. 1986 May;174(1):21-6. doi: 10.1016/0165-7992(86)90072-2.

Abstract

Hydrogen peroxide (H2O2) and caffeine were examined for their capacity for inducing SCEs and mutations at the HPRT locus in V79 Chinese hamster cells. Although, under standard conditions, both substances induced SCEs neither caused gene mutations. The SCE induction by both H2O2 and caffeine is influenced by BrdUrd substitution. Whereas H2O2 also induces lesions leading to SCEs in normal DNA, the SCe induction by caffeine depends on the replication of BrdUrd-substituted DNA. In cells with BrdUrd-substituted DNA, H2O2 induces mutations at the HPRT locus parallel to its SCE induction, whereas caffeine in the presence of BrdUrd only has an influence on the SCE rate. It is shown that the experimental conditions of the two test systems can play a decisive role when contradictory results are obtained.

摘要

研究了过氧化氢（H2O2）和咖啡因在V79中国仓鼠细胞中诱导姐妹染色单体交换（SCEs）以及在次黄嘌呤-鸟嘌呤磷酸核糖基转移酶（HPRT）基因座处诱导突变的能力。虽然在标准条件下，这两种物质都能诱导SCEs，但均未引起基因突变。H2O2和咖啡因对SCEs的诱导作用受5-溴脱氧尿苷（BrdUrd）替代的影响。H2O2还能诱导正常DNA中导致SCEs的损伤，而咖啡因对SCEs的诱导取决于BrdUrd替代DNA的复制。在含有BrdUrd替代DNA的细胞中，H2O2在诱导HPRT基因座突变的同时也诱导SCEs，而在BrdUrd存在的情况下，咖啡因仅对SCEs发生率有影响。结果表明，当获得相互矛盾的结果时，两个测试系统的实验条件可能起决定性作用。