Becerra Sandra C, Christy Barbara A, Herzig Maryanne C, Bynum James A, Darlington Daniel N
Pathology, Epidemiology and Biostatistics, United States Army Institute of Surgical Research, Fort Sam Houston, Texas, 78234, USA.
Blood and Shock Research, United States Army Institute of Surgical Research, Fort Sam Houston, Texas, 78234, USA.
Transfusion. 2023 May;63 Suppl 3:S199-S207. doi: 10.1111/trf.17345. Epub 2023 Apr 8.
The risk of military and civilian radiation exposure is increasing, and determining the effects of exposure is a high priority. Irradiation of the nearby blood supply after a nuclear event may impede mobilization of blood products for resuscitation at a time of great need. RBCs are administered to patients with trauma and hemorrhage to transport and deliver oxygen and avoid tissue hypoxia. Here we determine the effects of ionizing radiation on the energy metabolome of RBCs isolated from cold stored whole blood to determine if their stability is compromised by radiation exposure.
Whole blood from healthy volunteers was subjected to 0, 25, or 75 Gy of X-irradiation, and stored at 4°C. RBCs were isolated from stored WB at 0, 1, 7, 14, and 21 days of storage. The levels of extracted Krebs cycle intermediates, nicotinamide adenine dinucleotides, and phosphorylated derivatives of adenosine and guanosine were determined by tandem mass spectroscopy.
Irradiation at either 25Gy or 75Gy had no significant effect on any parameter measured compared to control (0Gy). However, there was a significant change over time in storage for ATP, GDP, and guanosine.
Irradiation at doses up to 75Gy had no effect on the energy metabolome of RBCs prepared from blood stored at 4°C for up to 21 days, suggesting that the RBC energy metabolome is not affected by radiation exposure and the blood can still be used for resuscitation in trauma patients.
军事和民用辐射暴露的风险正在增加，确定暴露的影响是当务之急。核事件后附近血液供应受到辐射可能会在急需时阻碍用于复苏的血液制品的动员。红细胞被用于创伤和出血患者，以运输和输送氧气并避免组织缺氧。在此，我们确定电离辐射对从冷藏全血中分离出的红细胞能量代谢组的影响，以确定其稳定性是否会因辐射暴露而受损。
将来自健康志愿者的全血接受0、25或75 Gy的X射线照射，并储存在4°C。在储存的第0、1、7、14和21天从储存的全血中分离红细胞。通过串联质谱法测定提取的三羧酸循环中间体、烟酰胺腺嘌呤二核苷酸以及腺苷和鸟苷的磷酸化衍生物的水平。
与对照组（0 Gy）相比，25 Gy或75 Gy的照射对所测量的任何参数均无显著影响。然而，ATP、GDP和鸟苷在储存过程中随时间有显著变化。
高达75 Gy的照射对在4°C储存长达21天的血液制备的红细胞能量代谢组没有影响，这表明红细胞能量代谢组不受辐射暴露影响，并且该血液仍可用于创伤患者的复苏。
