Mahmoud Shimaa A, El-Kosasy Amira M, Fouad Fatma A
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo 11754, Egypt.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo.
J Chromatogr Sci. 2023 Dec 13;61(10):907-917. doi: 10.1093/chromsci/bmad025.
Recently, the aim of analytical community is to reduce the usage of hazardous chemicals; so eco-friendly, rapid, selective and cost-effective methods were developed for simultaneous determination of montelukast sodium (MKT) and loratadine (LRT). The first method was based on chromatographic separation performed on precoated silica gel 60 GF254 plates with ethyl acetate-ethanol 9: 1 (v/v) as the mobile phase. The developed plates were scanned and quantified at 260 nm. The method gives linear correlation over concentration ranges of 0.3-3.6 μg/spot and 0.2-4.0 μg/spot for MKT and LRT, respectively. It was also successfully applied to analysis of both drugs in their pharmaceutical preparation and human plasma. The other methods are UV-spectrophotometric methods based on smart spectra manipulating to zero order spectrum of each drug. These methods are named response correlation (RC), a-centering and ratio derivative methods. RC and a-centering methods were dependent on the presence of an isosbestic point between the overlapped spectra of both drugs. While ratio derivative method based on manipulation of the ratio spectra of both drugs. The two drugs obey Beer-Lambert law over the concentration ranges of 3.0-30.0 μg/mL in the three spectrophotometric methods. Moreover, the greenness of the developed methods is assessed using suitable analytical Eco-Scale and Green Analytical Procedure Index.
最近，分析界的目标是减少有害化学品的使用；因此，开发了环保、快速、选择性强且具有成本效益的方法来同时测定孟鲁司特钠（MKT）和氯雷他定（LRT）。第一种方法基于在预涂硅胶60 GF254板上进行的色谱分离，以乙酸乙酯 - 乙醇9:1（v/v）作为流动相。展开后的薄板在260 nm处进行扫描和定量。该方法在MKT和LRT的浓度范围分别为0.3 - 3.6 μg/斑点和0.2 - 4.0 μg/斑点时给出线性相关性。它还成功应用于两种药物在其药物制剂和人血浆中的分析。其他方法是基于对每种药物的零级光谱进行智能光谱处理的紫外分光光度法。这些方法被称为响应相关性（RC）、α - 中心化和比率导数法。RC和α - 中心化方法依赖于两种药物重叠光谱之间存在等吸收点。而比率导数法基于对两种药物的比率光谱进行处理。在这三种分光光度法中，两种药物在3.0 - 30.0 μg/mL的浓度范围内符合比尔 - 朗伯定律。此外，使用合适的分析生态量表和绿色分析程序指数对所开发方法的绿色度进行评估。
