Shen Congle, Li Weijie, Wang Yajie
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
Transl Cancer Res. 2023 Mar 31;12(3):525-535. doi: 10.21037/tcr-22-1972. Epub 2023 Mar 20.
As an internal reference gene, glyceraldehyde-3-phosphate dehydrogenase () plays an important role in glycolysis. While increasing evidence suggests that plays a crucial role in tumorigenesis of some cancers, no systematic analysis of has been conducted. Here, we sought to analyze the expression of and its oncogenic processes in pan-cancer.
was investigated in The Cancer Genome Atlas (TCGA) tumor types using several bioinformatic tools including Tumor IMmune Estimation Resource (TIMER), Gene Expression Profiling Interactive Analysis (GEPIA), University of ALabama at Birmingham CANcer (UALCAN), cBio Cancer Genomics Portal (cBioPortal), and Search Tool for Recurring Instances of Neighbouring Genes (STRING) for the expression and relationships with prognosis and immune infiltration separately.
Through our analysis, we measured the higher expression of across the majority of TCGA tumors. overexpression predicts poor survival in patients with tumors expressing a high level of . Moreover, the genetic changes in contributed to an increased mRNA expression. Additionally, expression was negatively correlated with immune infiltration involving cancer-associated fibroblasts, neutrophil cell and endothelial.
The house-keeping gene might be a promising biomarker for pan-cancer prognosis. And is not suitable as an internal reference gene for most cancer research, whether RNA or protein analyses.
作为一种内参基因，甘油醛-3-磷酸脱氢酶（）在糖酵解中起重要作用。虽然越来越多的证据表明在某些癌症的肿瘤发生中起关键作用，但尚未对进行系统分析。在此，我们试图分析在泛癌中的表达及其致癌过程。
使用多种生物信息学工具，包括肿瘤免疫评估资源（TIMER）、基因表达谱交互式分析（GEPIA）、阿拉巴马大学伯明翰分校癌症（UALCAN）、cBio癌症基因组学门户（cBioPortal）以及邻近基因重复实例搜索工具（STRING），分别在癌症基因组图谱（TCGA）肿瘤类型中研究的表达以及与预后和免疫浸润的关系。
通过我们的分析，我们测量了大多数TCGA肿瘤中较高的表达水平。过表达预示着高表达肿瘤患者的不良生存。此外，的基因变化导致mRNA表达增加。此外，的表达与涉及癌症相关成纤维细胞、中性粒细胞和内皮细胞的免疫浸润呈负相关。
管家基因可能是泛癌预后的一个有前景的生物标志物。而且，无论是RNA还是蛋白质分析，都不适合作为大多数癌症研究的内参基因。
