Research on the oncogenic role of the house-keeping gene in human tumors.

BACKGROUND

As an internal reference gene, glyceraldehyde-3-phosphate dehydrogenase () plays an important role in glycolysis. While increasing evidence suggests that plays a crucial role in tumorigenesis of some cancers, no systematic analysis of has been conducted. Here, we sought to analyze the expression of and its oncogenic processes in pan-cancer.

METHODS

was investigated in The Cancer Genome Atlas (TCGA) tumor types using several bioinformatic tools including Tumor IMmune Estimation Resource (TIMER), Gene Expression Profiling Interactive Analysis (GEPIA), University of ALabama at Birmingham CANcer (UALCAN), cBio Cancer Genomics Portal (cBioPortal), and Search Tool for Recurring Instances of Neighbouring Genes (STRING) for the expression and relationships with prognosis and immune infiltration separately.

RESULTS

Through our analysis, we measured the higher expression of across the majority of TCGA tumors. overexpression predicts poor survival in patients with tumors expressing a high level of . Moreover, the genetic changes in contributed to an increased mRNA expression. Additionally, expression was negatively correlated with immune infiltration involving cancer-associated fibroblasts, neutrophil cell and endothelial.

CONCLUSIONS

The house-keeping gene might be a promising biomarker for pan-cancer prognosis. And is not suitable as an internal reference gene for most cancer research, whether RNA or protein analyses.