Donoso Valeria, Whitson Jeremy, Lom Barbara
Biology, Davidson College, Davidson, North Carolina, United States.
Preventative Medicine, Northwestern University, Chicago, Illinois, United States.
MicroPubl Biol. 2023 Mar 20;2023. doi: 10.17912/micropub.biology.000786. eCollection 2023.
The transcription factor Prdm12 exerts important influences on the development of nociceptors, peripheral touch and pain-sensing neurons, and has been implicated in human pain sensation disorders. We examined the consequences of exposing developing embryos to the commonly used pesticide malathion on Prdm12 expression. Using qPCR and western blot analysis we observed that malathion treatment for the first six days of tadpole development significantly increased both mRNA levels and Prdm12 protein levels compared to controls. Consequently, early exposure to this pesticide has potential to alter nociceptor development.
转录因子Prdm12对伤害感受器、外周触觉和痛觉神经元的发育具有重要影响，并且与人类疼痛感觉障碍有关。我们研究了发育中的胚胎暴露于常用杀虫剂马拉硫磷对Prdm12表达的影响。通过定量PCR和蛋白质免疫印迹分析，我们观察到，与对照组相比，在蝌蚪发育的前六天用马拉硫磷处理显著提高了Prdm12的mRNA水平和蛋白质水平。因此，早期接触这种杀虫剂有可能改变伤害感受器的发育。
