Veroli María Victoria, Lamitina Todd
Department of Pediatrics, University of Pittsburgh, Pittsburgh, Pennsylvania, United States.
MicroPubl Biol. 2023 Mar 22;2023. doi: 10.17912/micropub.biology.000818. eCollection 2023.
monitoring of gene expression using standard transcriptional reporters is a powerful and commonly used tool for genetic dissection of the osmotic stress response in . Like all transgene reporters, these reporters have important limitations that restrict their utility. To overcome these limitations, we created three different reporters using CRISPR/Cas9 methods to insert several variants of GFP into the endogenous locus. These new strains provide a more powerful and accurate tool for the analysis of regulatory pathways.
使用标准转录报告基因监测基因表达是对[具体物种]渗透胁迫反应进行遗传剖析的一种强大且常用的工具。与所有转基因报告基因一样，这些报告基因存在重要局限性，限制了它们的实用性。为克服这些局限性，我们使用CRISPR/Cas9方法创建了三种不同的报告基因，将几种绿色荧光蛋白（GFP）变体插入到内源性[具体基因]位点。这些新菌株为分析[具体基因]调控途径提供了一种更强大、更准确的工具。
