Thye Kah-Lok, Wan Abdullah Wan Muhamad Asrul Nizam, Ong-Abdullah Janna, Lamasudin Dhilia Udie, Wee Chien-Yeong, Mohd Yusoff Mohd Hafis Yuswan, Loh Jiun-Yan, Cheng Wan-Hee, Lai Kok-Song
Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia.
Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute, 43400 Serdang, Selangor Malaysia.
Physiol Mol Biol Plants. 2023 Mar;29(3):377-392. doi: 10.1007/s12298-023-01293-w. Epub 2023 Mar 10.
Utilisation of calcium lignosulfonate (CaLS) in has been reported to improve shoot multiplication. However, mechanisms responsible for such observation remain unknown. Here, we elucidated the underlying mechanisms of CaLS in promoting shoot multiplication of via comparative proteomics, biochemical assays, and nutrient analysis. The proteome profile of CaLS-treated plants showed enhancement of several important cellular metabolisms such as photosynthesis, protein synthesis, Krebs cycle, glycolysis, gluconeogenesis, and carbohydrate synthesis. Further biochemical analysis recorded that CaLS increased Rubisco activity, hexokinase activity, isocitrate dehydrogenase activity, total carbohydrate content, glutamate synthase activity and total protein content in plant shoot, suggesting the role of CaLS in enhancing shoot growth via upregulation of cellular metabolism. Subsequent nutrient analysis showed that CaLS treatment elevated the contents of several nutrient ions especially calcium and sodium ions. In addition, our study also revealed that CaLS successfully maintained the cellular homeostasis level through the regulation of signalling molecules such as reactive oxygen species and calcium ions. These results demonstrated that the CaLS treatment can enhance shoot multiplication in Andrews by stimulating nutrient uptake, inducing cell metabolism, and regulating cell homeostasis.
The online version contains supplementary material available at 10.1007/s12298-023-01293-w.
据报道，在[具体植物名称未给出]中使用木质素磺酸钙（CaLS）可提高芽增殖。然而，导致这种观察结果的机制尚不清楚。在此，我们通过比较蛋白质组学、生化分析和营养分析，阐明了CaLS促进[具体植物名称未给出]芽增殖的潜在机制。CaLS处理植物的蛋白质组图谱显示，光合作用、蛋白质合成、三羧酸循环、糖酵解、糖异生和碳水化合物合成等几种重要的细胞代谢得到增强。进一步的生化分析表明，CaLS增加了植物芽中核酮糖-1,5-二磷酸羧化酶/加氧酶（Rubisco）活性、己糖激酶活性、异柠檬酸脱氢酶活性、总碳水化合物含量、谷氨酸合酶活性和总蛋白质含量，表明CaLS通过上调细胞代谢在促进芽生长中发挥作用。随后的营养分析表明，CaLS处理提高了几种营养离子的含量，尤其是钙离子和钠离子。此外，我们的研究还表明，CaLS通过调节活性氧和钙离子等信号分子成功维持了细胞内稳态水平。这些结果表明，CaLS处理可以通过刺激养分吸收、诱导细胞代谢和调节细胞内稳态来增强[具体植物名称未给出]安德鲁斯的芽增殖。
在线版本包含可在10.1007/s12298-023-01293-w获取的补充材料。
