Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
Ningjin Hospital of Chinese Medicine, Shandong, China.
Biochem Genet. 2023 Dec;61(6):2348-2362. doi: 10.1007/s10528-023-10372-2. Epub 2023 Apr 10.
Previous studies found that the circadian clock gene participated in the genesis and development of breast cancer. However, research findings on the relationship between polymorphisms in the CLOCK gene and breast cancer risk were inconsistent. This study performed a meta-analysis of the association between CLOCK gene polymorphisms and breast cancer risk. PubMed, Cochrane Library, and Embase databases were electronically searched to collect studies on the association between CLOCK gene polymorphisms and breast cancer risk from inception to February 14, 2022. The quality of the included literature was assessed using the Newcastle-Ottawa Scale. For statistical analysis, odds ratio (OR) and 95% confidence intervals (CIs) were calculated using STATA 14.0. In addition, publication bias was performed by the funnel diagram and the Harbord's regression test. And sensitivity analysis was assessed by the trim and fill method. A total of 6 eligible studies, including 10,164 subjects (5488 breast cancer cases and 4676 controls), were screened in this meta-analysis. Though we did not find a significant association between the polymorphisms in the overall CLOCK gene with breast cancer risk [OR (95%CI) = 0.98 (0.96, 1.01), P = 0.148], we found that compared with T/T types of rs3749474 in CLOCK, T/C and C/C types of rs3749474 were associated with lower risk of breast cancer [OR (95%CI) = 0.93 (0.88, 0.98), P = 0.003]. The sensitivity analysis confirmed the robustness of the results. The funnel plot showed no significant publication bias. Polymorphisms in the CLOCK gene might be associated with breast cancer risk. More studies are needed to confirm the conclusion.
先前的研究发现，生物钟基因参与了乳腺癌的发生和发展。然而，关于 CLOCK 基因多态性与乳腺癌风险之间的关系的研究结果并不一致。本研究对 CLOCK 基因多态性与乳腺癌风险之间的关系进行了荟萃分析。通过电子检索 PubMed、Cochrane Library 和 Embase 数据库，收集了截至 2022 年 2 月 14 日关于 CLOCK 基因多态性与乳腺癌风险之间关系的研究。使用纽卡斯尔-渥太华量表评估纳入文献的质量。使用 STATA 14.0 计算优势比（OR）和 95%置信区间（CI）。此外，通过漏斗图和 Harbord 回归检验进行发表偏倚分析，并通过修剪和填充法评估敏感性分析。本荟萃分析共筛选出 6 项符合条件的研究，共纳入 10164 名受试者（5488 例乳腺癌病例和 4676 例对照）。虽然我们没有发现总体 CLOCK 基因多态性与乳腺癌风险之间存在显著关联[OR（95%CI）=0.98（0.96，1.01），P=0.148]，但我们发现与 CLOCK 中的 rs3749474 的 T/T 型相比，T/C 和 C/C 型的 rs3749474 与乳腺癌风险降低相关[OR（95%CI）=0.93（0.88，0.98），P=0.003]。敏感性分析证实了结果的稳健性。漏斗图显示无显著发表偏倚。CLOCK 基因多态性可能与乳腺癌风险相关。需要更多的研究来证实这一结论。
