Zhejiang Key Laboratory of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310000, China.
Plant Cell Physiol. 2023 Jun 15;64(6):686-699. doi: 10.1093/pcp/pcad031.
Reactive oxygen species (ROS) are highly reactive molecules, generated by nicotinamide adenine dinucleotide phosphate oxidases encoded by respiratory burst oxidase homologs. The functions of the OsRbohs gene family in rice are diverse and poorly understood. OsRbohI was recently identified as a newly evolved gene in the rice OsRbohs gene family. However, the function of OsRbohI in regulating rice growth is not yet reported. In this study, our results indicate that knockout (KO) OsRbohI mutants showed significantly shorter shoot and primary roots, along with lower ROS content than the control lines, whereas the overexpression (OE) lines displayed contrasting results. Further experiments showed that the abnormal length of the shoot and root is mainly caused by altered cell size. These results indicate that OsRbohI regulates rice shoot and root growth through the ROS signal. More importantly, RNA-seq analysis and jasmonic acid (JA) treatment demonstrated that OsRbohI regulates rice growth via the JA synthesis and signaling pathways. Compared with the control, the results showed that the KO mutants were more sensitive to JA, whereas the OE lines were less sensitive to JA. Collectively, our results reveal a novel pathway in which OsRbohI regulates rice growth and development by affecting their ROS homeostasis through JA synthesis and signaling pathway.
活性氧（ROS）是由烟酰胺腺嘌呤二核苷酸磷酸氧化酶（respiratory burst oxidase homologs）编码的高度反应性分子。水稻 OsRbohs 基因家族的功能多种多样，但了解甚少。OsRbohI 最近被鉴定为水稻 OsRbohs 基因家族中的一个新进化基因。然而，OsRbohI 在调节水稻生长中的功能尚未报道。在本研究中，我们的结果表明，敲除（KO）OsRbohI 突变体的茎和主根明显短于对照系，ROS 含量也较低，而过表达（OE）系则表现出相反的结果。进一步的实验表明，茎和根的异常长度主要是由于细胞大小的改变所致。这些结果表明，OsRbohI 通过 ROS 信号调节水稻的茎和根生长。更重要的是，RNA-seq 分析和茉莉酸（JA）处理表明，OsRbohI 通过 JA 合成和信号通路调节水稻生长。与对照相比，结果表明 KO 突变体对 JA 更敏感，而 OE 系对 JA 的敏感性降低。综上所述，我们的研究结果揭示了一条新的途径，即 OsRbohI 通过 JA 合成和信号通路影响其 ROS 稳态来调节水稻的生长和发育。
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