Molecular Biology and Biotechnology Division, Council of Scientific & Industrial Research-National Botanical Research Institute (CSIR-NBRI), Lucknow, 226001, India.
Molecular Biology and Biotechnology Division, Council of Scientific & Industrial Research-National Botanical Research Institute (CSIR-NBRI), Lucknow, 226001, India.
Ecotoxicol Environ Saf. 2020 Sep 15;201:110735. doi: 10.1016/j.ecoenv.2020.110735. Epub 2020 May 29.
Methyl jasmonate (Me-JA) is a plant growth regulator known for modulating plant responses to various abiotic and biotic stresses. The unavoidable arsenic (As) contamination in rice (Oryza sativa) results in reduced crop yield and greater carcinogenic risk to humans. The present work examines the significance of Me-JA induced molecular signaling and tolerance towards arsenic toxicity in rice. The arsenite (AsIII; 25 μM) stress hampered the overall growth and development of the rice seedling. However, the co-application (25 μM AsIII+0.25 μM Me-JA) resulted in increased biomass, chlorophyll content, enhanced antioxidant enzyme activities as compared to AsIII treated plants. The co-application also demonstrated a marked decrease in malondialdehyde content, electrolyte leakage and accumulation of total AsIII content (root + shoot) as compared to AsIII treated plants. The co-application also modulated the expression of genes involved in downstream JA signaling pathway (OsCOI, OsJAZ3, OsMYC2), AsIII uptake (OsLsi1, OsLsi2, OsNIP1;1, OsNIP3;1), translocation (OsLsi6, and OsINT5) and detoxification (OsNRAMP1, OsPCS2, and OsABCC2) which revealed the probable adaptive response of the rice plant to cope up arsenic stress. Our findings reveal that Me-JA alleviates AsIII toxicity by modulating signaling components involved in As uptake, translocation, and detoxification and JA signaling in rice. This study augments our knowledge for the future use of Me-JA in improving tolerance against AsIII stress.
茉莉酸甲酯（Me-JA）是一种植物生长调节剂，已知可调节植物对各种非生物和生物胁迫的反应。水稻中不可避免的砷（As）污染导致作物产量降低，对人类的致癌风险更大。本研究探讨了 Me-JA 诱导的分子信号转导对水稻砷毒性的重要性。亚砷酸盐（AsIII；25 μM）胁迫阻碍了水稻幼苗的整体生长和发育。然而，与单独用 AsIII 处理的植物相比，共处理（25 μM AsIII+0.25 μM Me-JA）导致生物量增加、叶绿素含量增加、抗氧化酶活性增强。与单独用 AsIII 处理的植物相比，共处理还表现出丙二醛含量、电解质泄漏和总 AsIII 含量（根+苗）的显著减少。共处理还调节了参与下游 JA 信号通路的基因的表达（OsCOI、OsJAZ3、OsMYC2）、AsIII 摄取（OsLsi1、OsLsi2、OsNIP1;1、OsNIP3;1）、转运（OsLsi6 和 OsINT5）和解毒（OsNRAMP1、OsPCS2 和 OsABCC2），这表明水稻植物可能对砷胁迫做出了适应反应。我们的研究结果表明，Me-JA 通过调节与 As 摄取、转运和解毒以及 JA 信号转导相关的信号成分，缓解了 AsIII 毒性。这项研究增加了我们对未来使用 Me-JA 提高水稻对 AsIII 胁迫耐受性的认识。
