Al Halnak A, Morliere P, Santus R, Nordmann Y, Dubertret L
Photodermatol. 1986 Apr;3(2):92-7.
We have studied the photosensitized oxidation, via singlet oxygen production, of histidine (His) and tryptophan (Trp) in the serum of porphyria patients and in the serum of healthy volunteers before or after addition of hematoporphyrin. It was observed that free plasma His and Trp are good probes of singlet oxygen production in the blood under visible light irradiation. However, Trp, which is mostly bound to serum albumin, is much less susceptible to photooxidation than His, which remains free in the plasma. These results must not be ignored in systemic effects of porphyrias and in photochemotherapy with hematoporphyrin derivatives.
我们研究了通过单线态氧生成，在卟啉症患者血清以及添加血卟啉前后的健康志愿者血清中，组氨酸（His）和色氨酸（Trp）的光敏氧化作用。据观察，游离血浆中的His和Trp是可见光照射下血液中单线态氧生成的良好探针。然而，大部分与血清白蛋白结合的Trp比在血浆中保持游离状态的His更不易受到光氧化作用的影响。在卟啉症的全身效应以及血卟啉衍生物的光化学疗法中，这些结果不容忽视。
