法国北部城市地区成年人的居住环境大气污染暴露与循环miRNA之间的关系。

The relationship between residential exposure to atmospheric pollution and circulating miRNA in adults living in an urban area in northern France.

作者信息

Hubert Audrey, Achour Djamal, Grare Céline, Zarcone Gianni, Muntaner Manon, Hamroun Aghiles, Gauthier Victoria, Amouyel Philippe, Matran Régis, Zerimech Farid, Lo-Guidice Jean-Marc, Dauchet Luc

机构信息

Univ. Lille, INSERM, CHU Lille, Institut Pasteur de Lille, U1167 - RID-AGE - Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement, F-59000 Lille, France.

Univ. Lille, CHU Lille, Institut Pasteur de Lille, ULR 4483 - IMPECS - IMPact de l'Environnement Chimique sur la Santé, F-59000 Lille, France.

出版信息

Environ Int. 2023 Apr;174:107913. doi: 10.1016/j.envint.2023.107913. Epub 2023 Apr 3.

DOI:10.1016/j.envint.2023.107913

PMID:37037173

Abstract

INTRODUCTION

MicroRNAs are epigenetic regulatory factors capable of silencing the expression of target genes and might mediate the effects of air pollution on health. The objective of the present population-based study was to investigate the association between microRNA expression and long-term, residential exposure to atmospheric PM and NO.

METHOD

We included 998 non-smoking adult participants from the cross-sectional ELISABET survey (2010-2014) in the Lille urban area of France. The mean residential annual pollution levels were estimated with an atmospheric dispersion modelling system. Ten microRNAs were selected on the basis of the literature data, together with two housekeeping microRNAs (miR-93-5p and miR-191-5p) and were quantified with RT-qPCRs. Multivariate linear regression models were used to study the association between microRNAs and air pollution. The threshold for statistical significance (after correction for the FDR) was set to p < 0.1.

RESULTS

The mean annual exposure between 2011 and the year of inclusion was 26.4 ± 2.0 µg/m for PM and 24.7 ± 5.1 µg/m for NO. Each 2 µg/m increment in PM exposure was associated with an 8.6% increment (95%CI [3.1; 14.3]; p = 0.019) in miR-451a expression. A 5 µg/m increment in NO exposure was associated with a 5.3% increment ([0.7; 10]; p = 0.056) in miR451a expression, a 3.6% decrement (95%CI [-6.1; -1.1]; p = 0.052) in miR-223-3p expression, a 3.8% decrement (95%CI[-6.8; -0.7]; p = 0.079) in miR-28-3p expression, a 4.3% decrement (95%CI [-7.7; -0.8]; p = 0.055) in miR-146a-5p expression, and a 4.0% decrement (95% CI[-7.4; -0.4]; p = 0.059) in miR-23a-5p expression. The difference between the two housekeeping microRNAs miR-93-5p and miR-191-5p was also associated with PM and NO exposure.

CONCLUSION

Our results suggest that circulating miRNAs are potentially valuable biomarkers of the effects of air pollution.

摘要

引言

微小RNA是能够使靶基因表达沉默的表观遗传调控因子，可能介导空气污染对健康的影响。本基于人群的研究目的是调查微小RNA表达与长期居住环境中大气颗粒物（PM）和氮氧化物（NO）暴露之间的关联。

方法

我们纳入了来自法国里尔市区横断面ELISABET调查（2010 - 2014年）的998名不吸烟成年参与者。使用大气扩散模型系统估算平均居住年度污染水平。根据文献数据选择了10种微小RNA，以及两种管家微小RNA（miR - 93 - 5p和miR - 191 - 5p），并通过逆转录定量聚合酶链反应（RT - qPCR）进行定量分析。使用多元线性回归模型研究微小RNA与空气污染之间的关联。经错误发现率（FDR）校正后的统计学显著性阈值设定为p < 0.1。

结果

2011年至纳入研究年份之间的平均年度暴露水平为，PM为26.4±2.0μg/m，NO为24.7±5.1μg/m。PM暴露每增加2μg/m，miR - 451a表达增加8.6%（95%置信区间[3.1；14.3]；p = 0.019）。NO暴露每增加5μg/m，miR - 451a表达增加5.3%（[0.7；10]；p = 0.056），miR - 223 - 3p表达减少3.6%（95%置信区间[-6.1；-1.1]；p = 0.052），miR - 28 - 3p表达减少3.8%（95%置信区间[-6.8；-0.7]；p = 0.079），miR - 146a - 5p表达减少4.3%（95%置信区间[-7.7；-0.8]；p = 0.055），miR - 23a - 5p表达减少4.0%（95%置信区间[-7.4；-0.4]；p = 0.059）。两种管家微小RNA miR - 93 - 5p和miR - 191 - 5p之间的差异也与PM和NO暴露有关。