Chen Dong, Zhao Zixi, Liu Peng, Liu Xinbang, Wang Xin, Ren Qiuyue, Chang Bai
Department of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.
NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, People's Republic of China.
Thromb Haemost. 2023 Oct;123(10):989-998. doi: 10.1055/s-0043-1768162. Epub 2023 Apr 10.
This study was conducted to assess the relationship between adventitial vasa vasorum neovascularization (VVn) in femoral artery of type 2 diabetic patients with macroangiopathy and the recruitment of macrophages and lymphocytes, and to relate the density of VVn to the occurrence of cardiovascular events.
Femoral artery samples were obtained from amputation cases. A total of 55 type 2 diabetic patients with macroangiopathy, 15 autopsy cases with type 2 diabetes without atherosclerosis.
Hematoxylin and eosin (H&E) staining to observe the histopathological features; Victoria blue staining to analyze the histological features; immunohistochemistry (CD34, CD68, CD20, and CD3) to determine the VVn density and the expression of macrophages, B lymphocytes, and T lymphocytes.
Type 2 diabetic patients with macroangiopathy showed a higher mean adventitial VVn density in femoral artery (48.40 ± 9.39 no./mm) than patients with type 2 diabetes without atherosclerosis (19.75 ± 6.28 no./mm) ( < 0.01). In addition, the VVn density was positively associated with the expression of CD68 macrophages ( = 0.62, < 0.01) and CD20 B lymphocytes ( = 0.59, < 0.01). Type 2 diabetic patients with high VVn density showed more adverse cardiovascular events (27/35 vs. 8/20 events, = 0.006). In multivariable analysis adjusted for main risk factors for cardiovascular disease, VVn was still independently associated with adverse cardiovascular events ( = 0.01).
VVn density in type 2 diabetic patients with macroangiopathy is positively correlated with the adventitial immune-inflammatory cell numbers and the development of atherosclerotic lesions. Furthermore, VVn density is associated with adverse cardiovascular events.
本研究旨在评估2型糖尿病合并大血管病变患者股动脉外膜血管新生（VVn）与巨噬细胞和淋巴细胞募集之间的关系，并将VVn密度与心血管事件的发生相关联。
股动脉样本取自截肢病例。共55例2型糖尿病合并大血管病变患者，15例2型糖尿病无动脉粥样硬化的尸检病例。
苏木精-伊红（H&E）染色观察组织病理学特征；维多利亚蓝染色分析组织学特征；免疫组织化学（CD34、CD68、CD20和CD3）测定VVn密度及巨噬细胞、B淋巴细胞和T淋巴细胞的表达。
2型糖尿病合并大血管病变患者股动脉外膜平均VVn密度（48.40±9.39个/mm）高于2型糖尿病无动脉粥样硬化患者（19.75±6.28个/mm）（P<0.01）。此外，VVn密度与CD68巨噬细胞（r=0.62，P<0.01）和CD20 B淋巴细胞（r=0.59，P<0.01）的表达呈正相关。VVn密度高的2型糖尿病患者发生更多不良心血管事件（27/35例与8/20例事件，P=0.006）。在针对心血管疾病主要危险因素进行校正的多变量分析中，VVn仍与不良心血管事件独立相关（P=0.01）。
2型糖尿病合并大血管病变患者的VVn密度与外膜免疫炎症细胞数量及动脉粥样硬化病变的发展呈正相关。此外，VVn密度与不良心血管事件相关。
