Department of Pharmacognosy, College of Pharmaceutical Sciences, Matsuyama University, Matsuyama, Ehime, Japan.
Biosci Biotechnol Biochem. 2023 Jun 23;87(7):688-695. doi: 10.1093/bbb/zbad043.
Hazelnuts contain biologically active phenolic compounds and are widely used for their nutritional value. In this study, the phenolic compounds contained in hazelnuts were isolated from the kernels of Corylus avellana L. and investigated. Spectral analyses revealed 2 new acetophenone glycosides, characterized as 2',4',6'-trihydroxyacetophenone-4'-O-(2-O-β-d-apiosyl)-β-d-glucoside and 2',4',6'-trihydroxyacetophenone-4'-O-(2-O-β-d-apiosyl-6-O-α-l-arabinosyl)-β-d-glucoside, and 4 known compounds. Four high-molecular-mass condensed tannin fractions were detected in the water-soluble fraction of the extract, characterized as B-type procyanidin consisting of extension and terminal units. Gel permeation chromatography analyses revealed that the average molecular mass, based on the polystyrene standard, was approximately 15 000-113 000. These high-molecular-mass condensed tannin fractions were chemically characterized and exhibited different molecular weights. The fractions of high-molecular-mass condensed tannins were obtained from hazelnuts and tested for 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. The EC50 values indicated significant activity for all the fractions.
榛子含有生物活性酚类化合物，因其营养价值而被广泛应用。在这项研究中，从榛子的果仁中分离并研究了榛子中含有的酚类化合物。光谱分析显示了 2 种新的苯乙酮糖苷，分别为 2'，4'，6'-三羟基苯乙酮-4'-O-(2-O-β-d-芹糖基)-β-d-葡萄糖苷和 2'，4'，6'-三羟基苯乙酮-4'-O-(2-O-β-d-芹糖基-6-O-α-l-阿拉伯糖基)-β-d-葡萄糖苷，以及 4 种已知的化合物。在提取物的水溶性部分检测到 4 种高分子量的缩合单宁分数，其特征为 B 型原花青素，由延伸和末端单元组成。凝胶渗透色谱分析表明，基于聚苯乙烯标准，其平均分子量约为 15000-113000。这些高分子量缩合单宁分数进行了化学表征，并表现出不同的分子量。从榛子中获得了高分子量缩合单宁的分数，并对 1,1-二苯基-2-苦基肼自由基清除活性进行了测试。EC50 值表明所有分数均具有显著的活性。
